FB2024_03 , released June 25, 2024
Allele: Hsap\TARDBPUAS.cVBa
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General Information
Symbol
Hsap\TARDBPUAS.cVBa
Species
H. sapiens
Name
FlyBase ID
FBal0320786
Feature type
allele
Associated gene
Hsap\TARDBP
Associated Insertion(s)
Carried in Construct
P{UAS-hTARDBP.WT}
Key Links
Transgenic product class
wild_type
(Vanden Broeck et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Vanden Broeck et al., 2015)
Mutagen
in vitro construct
(Vanden Broeck et al., 2015)
Progenitor genotype
Carried in construct
P{UAS-hTARDBP.WT}
Cytology
Description

UASt regulatory sequences drive expression of wild-type Hsap\TARDBP.

(Vanden Broeck et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\TARDBP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  amyotrophic lateral sclerosis type 10
      modeled by TBPHΔ142, TBPHΔ23
      (Vanden Broeck et al., 2015)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Hsap\TARDBPScer\UAS.cVBa driven by Scer\GAL4GMR.PU does not induce a rough eye phenotype and expression driven by Scer\GAL4nSyb.PU or Scer\GAL4elav-C155 does not cause wing expansion defects. With expression driven by Scer\GAL4nSyb.PU, there is no significant or progressive loss of Kenyon cells or changes in the length or thickness of the alpha and beta lobes in 3 or 50 day old flies, compared to controls. Expression driven by Scer\GAL4CCAP.PP results in predominantly nuclear localization of the protein, comparable with wild type.

      (Vanden Broeck et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Hsap\TARDBPG287S.Scer\UAS.cVBa, Hsap\TARDBPA315T.Scer\UAS.cVBa, Hsap\TARDBPD169G.Scer\UAS.cVBa, Hsap\TARDBPA90V.Scer\UAS.cVBa or Hsap\TARDBPScer\UAS.cVBa driven by Scer\GAL4nSyb.PU equally partially rescue pupal lethality (around 50% of flies expected by a full rescue eclose) in TBPHΔ142/TBPHΔ23 mutants. However, the median lifespan of surviving TBPHΔ142/TBPHΔ23 flies with expression of Hsap\TARDBPScer\UAS.cVBa is shorter than in controls. Expression (driven by Scer\GAL4nSyb.PU) of Hsap\TARDBPD169G.Scer\UAS.cVBa or Hsap\TARDBPScer\UAS.cVBa (but not Hsap\TARDBPA90V.Scer\UAS.cVBa, Hsap\TARDBPA315T.Scer\UAS.cVBa or Hsap\TARDBPG287S.Scer\UAS.cVBa) suppresses the loss of bursicon neurons seen in TBPHΔ142/TBPHΔ23 mutants.

      (Vanden Broeck et al., 2015)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Hsap\TARDBPScer\UAS.cVBa
      Hsap\TARDBPUAS.cVBa
      TARDBPWT
      (Vanden Broeck et al., 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)