FB2024_03 , released June 25, 2024
Allele: Dmel\robo1ΔIg1.UAS.Tag:HA,Tag:SS(wg)
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General Information
Symbol
Dmel\robo1ΔIg1.UAS.Tag:HA,Tag:SS(wg)
Species
D. melanogaster
Name
FlyBase ID
FBal0320371
Feature type
allele
Associated gene
Dmel\robo1
Associated Insertion(s)
Carried in Construct
M{10xUAS-HA-robo1.ΔIg1}
Key Links
Transgenic product class
inframe_deletion
(Brown et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Brown et al., 2015)
Mutagen
in vitro construct
(Brown et al., 2015)
Progenitor genotype
Carried in construct
M{10xUAS-HA-robo1.ΔIg1}
Cytology
Description

Ten copies of the UAS regulatory sequence drive expression of robo1 amino acid residues 153-1395 (lacks the Ig1 domain) tagged at the N-terminal end with three copies of the Tag:HA tag. 5'UTR and signal sequences from the wg gene are also present.

(Brown et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
robo1
Tagged with
Tag:HA
Tag:SS(wg)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of robo1ΔIg1.Scer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4elav.PLu in embryos does not result in defects in midline crossing in the ventral nerve cord.

      Expression of robo110xScer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4eg-Mz360 in embryos does not result in any defects in EW neuron midline crossing of the ventral nerve cord.

      (Brown et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      robo1ΔIg1.UAS.Tag:HA,Tag:SS(wg)/Scer\GAL4elav.PLu fails to rescue robo11

      (Brown et al., 2015)
      Comments

      Expression of robo1ΔIg1.Scer\UAS.T:Ivir\HA1,T:SS-wg under the control of Scer\GAL4elav.PLu does not rescue the FasII-positive axon guidance defects of robo11/robo11 mutants.

      (Brown et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      robo1ΔIg1.Scer\UAS.T:Ivir\HA1,T:SS-wg
      robo1ΔIg1.UAS.Tag:HA,Tag:SS(wg)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)