FlyBase Allele Report: Dmel\Sox21a[UAS.cCa]

General Information

Symbol

Dmel\Sox21a^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Chen

FlyBase ID

FBal0319974

Feature type

allele

Associated gene

Dmel\Sox21a

Associated Insertion(s)

Carried in Construct

P{UAS-Sox21a.C}

Also Known As

UAS-Sox21a

Key Links

Transgenic product class

wild_type

(Chen et al., 2016)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Chen et al., 2016)

Mutagen

in vitro construct

(Chen et al., 2016)

Progenitor genotype

Carried in construct

P{UAS-Sox21a.C}

Cytology

Description

UASt regulatory sequences drive expression of Sox21a.

(Chen et al., 2016)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Sox21a

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates intestinal cancer

modeled by Sox21a^JC1

(Chen et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | adult stage, with Scer\GAL4^Su(H).GBE

(Jin et al., 2020)

Phenotype Manifest In

adult midgut enterocyte | decreased number, with Scer\GAL4^Su(H).GBE

(Jin et al., 2020)

enteroblast | adult stage | increased number, with Scer\GAL4^Su(H).GBE

(Jin et al., 2020)

enterocyte, with Scer\GAL4^Su(H).GBE

(Chen et al., 2016)

Detailed Description

Statement

Reference

Expression of Sox21a^UAS.cCa under the control of Scer\GAL4^Su(H).GBE leads to adult midgut EBs to fail to differentiate into ECS.

(Jin et al., 2020)

Expression of Sox21a^Scer\UAS.cCa under the control of Scer\GAL4^Su(H).GBE induces enterocyte differentiation, as shown by cells becoming polyploid.

Somatic clones that include intestinal stem cells expressing Sox21a^Scer\UAS.cCa under the control of Scer\GAL4^unspecified grow normally in size and produce both polyploid enterocytes and enteroendocrine cells.

(Chen et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^Su(H).GBE, Sox21a^UAS.cCa has abnormal cell number | adult stage phenotype, non-suppressible by Sox100B^UAS.cJa/Scer\GAL4^Su(H).GBE

(Jin et al., 2020)

Suppressor of

Statement

Reference

Scer\GAL4^Su(H).GBE/Sox21a^UAS.cCa is a suppressor of abnormal cell number | adult stage phenotype of Scer\GAL4^Su(H).GBE, Sox100B^GD4588

(Jin et al., 2020)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^Su(H).GBE, Sox21a^UAS.cCa has enteroblast | increased number | adult stage phenotype, non-suppressible by Sox100B^UAS.cJa/Scer\GAL4^Su(H).GBE

(Jin et al., 2020)

Scer\GAL4^Su(H).GBE, Sox21a^UAS.cCa has adult midgut enterocyte | decreased number phenotype, non-suppressible by Sox100B^UAS.cJa/Scer\GAL4^Su(H).GBE

(Jin et al., 2020)

Suppressor of

Statement

Reference

Scer\GAL4^Su(H).GBE/Sox21a^UAS.cCa is a suppressor of enteroblast | increased number | adult stage phenotype of Scer\GAL4^Su(H).GBE, Sox100B^GD4588

(Jin et al., 2020)

Scer\GAL4^Su(H).GBE/Sox21a^UAS.cCa is a suppressor of adult midgut enterocyte | decreased number phenotype of Scer\GAL4^Su(H).GBE, Sox100B^GD4588

(Jin et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^Su(H).GBE/Sox21a^UAS.cCa partially rescues Sox21a^JC1

(Chen et al., 2016)

Fails to rescue

Scer\GAL4^{Delta-05151-G}/Sox21a^UAS.cCa fails to rescue Sox21a^JC1

(Chen et al., 2016)

Comments

Expression of Sox21a^Scer\UAS.cCa under the control of Scer\GAL4^Su(H).GBE (along with GAL80ts to temporally control expression), but not Scer\GAL4^Dl-05151-G, largely suppresses tumor formation of Sox21a^JC1/Sox21a^JC1.

Expression of Sox21a^Scer\UAS.cCa under the control of Scer\GAL4^Su(H).GBE rescues the intestinal stem cell overproliferation phenotype in Sox21a^JC1/Sox21a^JC1 mutant intestines.

(Chen et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

68156

w^*; P{UAS-Sox21a.C}8

68155

w^*; P{UAS-Sox21a.C}15/CyO

Notes on Origin

Discoverer