FB2024_03 , released June 25, 2024
Allele: Dmel\shvUAS.Tag:HA
Open Close
General Information
Symbol
Dmel\shvUAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0319441
Feature type
allele
Associated gene
Dmel\shv
Associated Insertion(s)
Carried in Construct
P{UAS-shv.HA}
Also Known As
UAS-shv
Key Links
Transgenic product class
wild_type
(Lee et al., 2016)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Lee et al., 2016)
Mutagen
in vitro construct
(Lee et al., 2017, Lee et al., 2016)
Progenitor genotype
Carried in construct
P{UAS-shv.HA}
Cytology
Description

UAS regulatory sequences drive expression of shv tagged with Tag:HA.

(Lee et al., 2017)

UAS regulatory sequences drive expression of the full-length shv coding region.

(Lee et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
shv
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of shvScer\UAS.T:Ivir\HA1 under the control of either Scer\GAL4nSyb.PS or Scer\GAL4how-24B does not perturb synaptic growth as the number of boutons as well as immature, undifferentiated 'ghost boutons' on the neuromuscular junctions in third instar larvae is comparable to controls. Scer\GAL4nSyb.PS-driven expression of shvScer\UAS.T:Ivir\HA1 also has no significant effect on the amplitude of either miniature or evoked excitatory postsynaptic potentials.

      (Lee et al., 2017)

      Expression of shvScer\UAS.cLa driven in hub and cyst cells by Scer\GAL4C833 does not significantly affect hub cell number (up to 35 days old) but does significantly decrease germline stem cell number (at 3, 7, 14, 35 and 50 days post eclosion) in the testes compared to controls. Expression of shvScer\UAS.cLa driven in hub cells by Scer\GAL4upd1.PU does not significantly affect hub cell number (up to 35 days old) in the testes compared to controls. Expression of shvScer\UAS.cLa driven in germ cells by Scer\GAL4nos.UTR.T:Hsim\VP16 does not significantly affect the number of germline stem cells per testes (up to 35 days old) but does significantly increase the number of hub cells at 3 and 50 days post eclosion (but not 7, 14 or 35 days) compared to controls.

      Expression of shvScer\UAS.cLa driven by Scer\GAL4C833, Scer\GAL4nos.UTR.T:Hsim\VP16 or Scer\GAL4upd1.PU increases hub cell number per testes in 50 day old flies compared to controls. Expression of shvScer\UAS.cLa driven by Scer\GAL4upd1.PU increases germline stem cell number per testes in 50 day old flies compared to controls.

      (Lee et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      NOT Suppressor of
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      shvUAS.Tag:HA/Scer\GAL4nSyb.PS is a suppressor of NMJ bouton | third instar larval stage phenotype of mysts1

      (Lee et al., 2017)
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4how-24B/shvUAS.Tag:HA is a non-suppressor of NMJ bouton | third instar larval stage phenotype of mysts1

      (Lee et al., 2017)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of shvScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4nSyb.PS (but not Scer\GAL4how-24B) rescues the increased number of boutons on neuromuscular junctions characteristic for mysts1 mutant third instar larvae.

      (Lee et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      shvUAS.Tag:HA/Scer\GAL4nSyb.PS rescues shv1

      (Lee et al., 2017)

      shvUAS.Tag:HA/Scer\GAL4C833 rescues shv1

      (Lee et al., 2016)

      Scer\GAL4C587/shvUAS.Tag:HA rescues shv1

      (Lee et al., 2016)
      Partially rescues

      Scer\GAL4VP16.nanos.UTR/shvUAS.Tag:HA partially rescues shv1

      (Lee et al., 2016)
      Fails to rescue

      Scer\GAL4how-24B/shvUAS.Tag:HA fails to rescue shv1

      (Lee et al., 2017)

      shvUAS.Tag:HA/Scer\GAL4upd1.PU fails to rescue shv1

      (Lee et al., 2016)
      Comments

      Expression of shvScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4nSyb.PS (but not Scer\GAL4how-24B) rescues the increased number of boutons on neuromuscular junctions as well as the decreased miniature excitatory postsynaptic potential amplitude observed in shv1 mutant third instar larvae.

      (Lee et al., 2017)

      Expression of shvScer\UAS.cLa driven in hub and cyst cells by Scer\GAL4C833 rescues hub integrity and germline stem cell number (at all ages tested), along with rescuing hub mislocalization, in shv1/shv1 testes. Expression of shvScer\UAS.cLa driven by Scer\GAL4C587 rescues hub integrity and germline stem cell number in shv1/shv1 testes. Expression of shvScer\UAS.cLa driven in hub cells by Scer\GAL4upd1.PU fails to rescue and indeed exacerbates hub integrity in shv1/shv1 testes (at all ages tested). Expression of shvScer\UAS.cLa driven in germ cells by Scer\GAL4nos.UTR.T:Hsim\VP16 partially preserves the hub (at all ages) and protects against decreases in germline stem cell number (except by day 35) in shv1/shv1 testes.

      (Lee et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      shvScer\UAS.T:Ivir\HA1
      shvScer\UAS.cLa
      shvUAS.Tag:HA
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Lee
      Secondary FlyBase IDs
        References (2)