FB2024_03 , released June 25, 2024
Allele: Dmel\Rab3N134I.UAS.cCa
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General Information
Symbol
Dmel\Rab3N134I.UAS.cCa
Species
D. melanogaster
Name
FlyBase ID
FBal0319086
Feature type
allele
Associated gene
Dmel\Rab3
Associated Insertion(s)
Carried in Construct
M{UAS-Rab3.N134I}
Key Links
Transgenic product class
dominant_negative_variant
(Chen et al., 2015)
missense_variant
(Chen et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Chen et al., 2015)
missense_variant
(Chen et al., 2015)
Mutagen
in vitro construct
(Chen et al., 2015)
Progenitor genotype
Carried in construct
M{UAS-Rab3.N134I}
Cytology
Description

UASt regulatory sequences drive expression of a mutant form of Rab3 which carries the amino acid replacement N134I. This is predicted to result in a GTP-binding deficient protein. The protein acts as a dominant-negative.

(Chen et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Rab3
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Unlike wild type (almost all GluRIIC clusters, which localize opposite release sites, are apposed to brp, which marks presynaptic active zones), only around two thirds of GluRIIC clusters are apposed to brp and active zones (brp area) are significantly larger the neuromuscular junction (NMJ) of third instar larvae (more posterior segments, 5 and 6) with expression of Rab3N134I.Scer\UAS.cCa driven by Scer\GAL4elav.Switch.PO (females maintained on fly food with RU486 for 2 days before mating and egg-laying on RU486 food).

      (Chen et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      Scer\GAL4VGlut.PD/Rab3N134I.UAS.cCa fails to rescue Df(2R)ED2076/Rab3rup

      (Chen et al., 2015)
      Comments

      Expression of Rab3N134I.Scer\UAS.cCa driven by Scer\GAL4VGlut.PD does not rescue active zone defects at the NMJ of Rab3rup/Df(2R)ED2076 third instar larvae.

      (Chen et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Rab3N134I.Scer\UAS.cCa
      Rab3N134I.UAS.cCa
      Name Synonyms
      Secondary FlyBase IDs
        References (1)