FlyBase Allele Report: Hsap\NUMB[UAS.PTBS-PRRL]

General Information

Symbol

Hsap\NUMB^{UAS.PTBS-PRRL}

Species

H. sapiens

Name

FlyBase ID

FBal0318529

Feature type

allele

Associated gene

Hsap\NUMB

Associated Insertion(s)

Carried in Construct

P{UAS-hNUMB.PTBS-PRRL}

Key Links

Transgenic product class

wild_type

(Verdi et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Verdi et al., 1999)

Mutagen

in vitro construct

(Verdi et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-hNUMB.PTBS-PRRL}

Cytology

Description

UASt regulatory sequences drive expression of the complete open reading frame of the Hsap\NUMB 'PTB[S]PRR[L]' alternative isoform (isoform 3), which contains the short version of the PTB domain and the long version of the PRR domain (contains a 48 amino acid residue insertion relative to the short form).

(Verdi et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\NUMB

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle | somatic clone | third instar larval stage

(Toriya et al., 2006)

abnormal neuroanatomy | somatic clone | third instar larval stage

(Toriya et al., 2006)

increased cell number | somatic clone | third instar larval stage

(Toriya et al., 2006)

visible, with Scer\GAL4^bbg-C96

(Verdi et al., 1999)

Phenotype Manifest In

Detailed Description

Statement

Reference

Compared to control clones in third instar larval brains, clones with expression of Hsap\NUMB^{Scer\UAS.PTBS-PRRL} have an increased number of neuroepithelial cells and neuroblasts contain more neurons.

(Toriya et al., 2006)

Overexpression of Hsap\NUMB^{Scer\UAS.PTBL-PRRS} (or Hsap\NUMB^{Scer\UAS.PTBS-PRRS}) leads to dramatic wing margin balding (much more severe than Hsap\NUMB^{Scer\UAS.PTBS-PRRL}), when driven by Scer\GAL4^bbg-C96, compared to controls; this represents a transformation toward neuronal fate.

(Verdi et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/Hsap\NUMB^{UAS.PTBS-PRRL} is a suppressor of abnormal neuroanatomy | somatic clone | third instar larval stage phenotype of numb¹⁵

(Toriya et al., 2006)

Scer\GAL4^Tub.PU/Hsap\NUMB^{UAS.PTBS-PRRL} is a suppressor of decreased cell number | somatic clone | third instar larval stage phenotype of numb¹⁵

(Toriya et al., 2006)

NOT Suppressor of

Statement

Reference

Hsap\NUMB^{UAS.PTBS-PRRL} is a non-suppressor of decreased cell number | somatic clone | third instar larval stage phenotype of N^ICN.UAS

(Toriya et al., 2006)

Other

Statement

Reference

Hsap\NUMB^{UAS.PTBS-PRRL}, N^ICN.UAS has increased cell number | somatic clone | third instar larval stage phenotype

(Toriya et al., 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/Hsap\NUMB^{UAS.PTBS-PRRL} is a suppressor of neuron | somatic clone | third instar larval stage phenotype of numb¹⁵

(Toriya et al., 2006)

NOT Suppressor of

Statement

Reference

Hsap\NUMB^{UAS.PTBS-PRRL} is a non-suppressor of neuron | somatic clone | third instar larval stage phenotype of N^ICN.UAS

(Toriya et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\NUMB^{Scer\UAS.PTBS-PRRL} increases the number of neuroepithelial cells but does not suppress the inhibition of neuronal differentiation seen in clones with expression of N^ICN.Scer\UAS in third instar larval neuroblasts.

Expression of Hsap\NUMB^{Scer\UAS.PTBS-PRRL} driven by Scer\GAL4^tub.PU partially suppresses neuronal differentiation defects seen in the outer optic anlage of numb¹⁵/numb¹⁵ clones in third instar larvae.

(Toriya et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\NUMB^{Scer\UAS.PTBS-PRRL}

Hsap\NUMB^{UAS.PTBS-PRRL}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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