FlyBase Allele Report: Dmel\CPT2[δ3]

General Information

Symbol

Dmel\CPT2^δ3

Species

D. melanogaster

Name

FlyBase ID

FBal0318504

Feature type

allele

Associated gene

Dmel\CPT2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Schulz et al., 2015)

Mutagen

P-element activity

(Schulz et al., 2015)

Progenitor genotype

P{GSV7}GS21694

(Schulz et al., 2015)

Cytology

Description

Imprecise excision of the progenitor resulting in a 3224bp deletion of the 5' UTR and the first 505 amino acids of the CPT2 ORF.

(Schulz et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:2,979,475..2,982,698 [+]

Comment:

Estimated extent of a 3224 bp deletion resulting from the imprecise excision of P{GSV7}GS21694. The excision is reported to include the 5' UTR and 505aa (methods) or 508aa (results) of the CPT2 coding sequence. This mapping assumes 505 codons have been completely removed.

(Schulz et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal starvation stress response | adult stage

(Schulz et al., 2015)

majority die during immature adult stage

(Schulz et al., 2015)

partially lethal - majority die

(Schulz et al., 2015)

partially lethal - majority die (with CPT2^δ18)

(Schulz et al., 2015)

partially lethal - majority die (with Df(3L)BSC428)

(Schulz et al., 2015)

partially lethal - majority die (with Df(3L)BSC671)

(Schulz et al., 2015)

short lived

(Schulz et al., 2015)

Phenotype Manifest In

adult brain

(Schulz et al., 2015)

flight muscle cell

(Schulz et al., 2015)

lipid droplet

(Schulz et al., 2015)

Detailed Description

Statement

Reference

The majority of CPT2^δ3 animals die during the first 3 days after eclosion - very few die during larval or pupal development. Those that survive the immediate post-eclosion period show a severely reduced adult lifespan and are hyper-sensitive to starvation.

Normally fed CPT2^δ3 flies are not heaver, but even lighter than controls. Body length is somewhat reduced, but the ratio of weight and size (BMI) is not increased, compared to controls. Motor activity is also unchanged. However, while total body triacylglyceride (TAG) levels are not increased, there is massive accumulation of TAG-filled lipid droplets in the brains and flight muscle in these mutant flies.

(Schulz et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

CPT2^δ3/CPT2^δ18 has partially lethal - majority die phenotype, suppressible by Scer\GAL4^da.PU/Rnor\Cpt2^UAS.cSa

(Schulz et al., 2015)

CPT2^δ3 has short lived phenotype, suppressible by Scer\GAL4^da.PU/Rnor\Cpt2^UAS.cSa

(Schulz et al., 2015)

CPT2^δ3 has short lived phenotype, suppressible | partially by Scer\GAL4^repo.PU/Rnor\Cpt2^UAS.cSa

(Schulz et al., 2015)

NOT suppressed by

Statement

Reference

CPT2^δ3/CPT2^δ18 has partially lethal - majority die phenotype, non-suppressible by Scer\GAL4^da.PU/Rnor\Cpt2^H372A.UAS

(Schulz et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

CPT2^δ3 is rescued by Scer\GAL4^nrv2.PU/CPT2^UAS.cSa

(Schulz et al., 2015)

CPT2^δ3 is rescued by CPT2^UAS.cSa/Scer\GAL4^repo.PU

(Schulz et al., 2015)

CPT2^δ3 is rescued by CPT2^UAS.cSa/Scer\GAL4^Mef2.PU

(Schulz et al., 2015)

CPT2^δ3 is rescued by CPT2^UAS.cSa/Scer\GAL4^NP2222

(Schulz et al., 2015)

CPT2^δ3 is rescued by CPT2^UAS.cSa/Scer\GAL4^Eaat1.PU

(Schulz et al., 2015)

Not rescued by

CPT2^δ3 is not rescued by CPT2^UAS.cSa/Scer\GAL4^Cg.PU

(Schulz et al., 2015)

CPT2^δ3 is not rescued by Scer\GAL4^nSyb.PU/CPT2^UAS.cSa

(Schulz et al., 2015)

CPT2^δ3 is not rescued by CPT2^UAS.cSa/Scer\GAL4^NP6520

(Schulz et al., 2015)

Comments

Glial-specific (Scer\GAL4^repo.PU) expression of CPT2^Scer\UAS.cSa reverses brain TAG accumulation and peri-eclosion lethality of CPT2^δ3 mutants.

Muscle-specific (Scer\GAL4^Mef2.PU), but not fat body (Scer\GAL4^cg.PU) or neuronal (Scer\GAL4^nSyb.PU), expression of CPT2^Scer\UAS.cSa reverses peri-eclosion lethality of CPT2^δ3 mutants.

Expression of CPT2^Scer\UAS.cSa in cortex glia (Scer\GAL4^{Akap200-NP2222} or Scer\GAL4^nrv2.PU) or astrocyte-like glia (Scer\GAL4^Eaat1.PU), but not in ensheathing glia (Scer\GAL4^NP6520), reverses peri-eclosion lethality of CPT2^δ3 mutants.

(Schulz et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CPT2^δ3

dCPT2^δ3

(Schulz et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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