FB2024_03 , released June 25, 2024
Allele: Dmel\obe1
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General Information
Symbol
Dmel\obe1
Species
D. melanogaster
Name
FlyBase ID
FBal0318085
Feature type
allele
Associated gene
Dmel\obe
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

hypomorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - molecular evidence

(Vichas et al., 2015)
Mutagen
spontaneous
(Vichas et al., 2015)
Progenitor genotype
Cytology
Description

No mutation has been identified in the exons or splice sites of obe transcription unit. However, the full length transcript is expressed at 10% of wild-type levels in the progeny of homozygous females.

(Vichas et al., 2015)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos derived from homozygous females show significantly reduced axis elongation during germband extension compared to wild type (the severity of the defect is unaffected by the paternal obe contribution).

      Embryos derived from obe1/obe2 females show significantly reduced axis elongation during germband extension compared to wild type (the severity of the defect is unaffected by the paternal obe contribution).

      obe1/Df(3R)Exel6174 embryos derived from obe1/Df(3R)Exel6174 females crossed to obe1/+ males show significantly reduced axis elongation during germband extension compared to wild type.

      Homozygous obe1 embryos derived from homozygous females crossed to heterozygous males and obe1/obe2 embryos derived from obe1/obe2 females crossed to obe2/+ males show an abnormal aggregation of adherens junction proteins at stage 7.

      In contrast to wild type, most of the centrosomes in epithelial cells fail to dissociate from microtubules and remain in close proximity to adherens junctions throughout axis elongation (stages 6 and 7) in obe1/obe2 embryos derived from obe1/obe2 females crossed to obe2/+ males. This results in a failure of the centrosomes to relocalise laterally. The mis-positioned centrosomes are often associated with adherens junction aggregates.

      The adherens junction and centrosome defects seen in stage 7 obe1/obe2 embryos derived from obe1/obe2 females crossed to obe2/+ males are rescued by nocodazole treatment (which inhibits microtubule polymerisation).

      (Vichas et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      obe2

      (Vichas et al., 2015)
      Rescued by

      obe1 is rescued by obeCH322-15H13

      (Vichas et al., 2015)

      obe1 is rescued by obesqh.PV

      (Vichas et al., 2015)

      obe1/obe2 is rescued by obeCH322-15H13

      (Vichas et al., 2015)

      obe1/obe2 is rescued by obesqh.PV

      (Vichas et al., 2015)
      Comments

      Expression of obeCH322-15H13 rescues the axis elongation defects seen during germband extension in the progeny of obe1/obe1 females crossed to obe1/+ males.

      Expression of obesqh.PV rescues the axis elongation defects seen during germband extension in the progeny of obe1/obe1 females crossed to obe1/+ males.

      (Vichas et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      Isolated in the background of a subset of PBac{SAstopDsRed} insertion lines on chromosome arm 3R.

      (Vichas et al., 2015)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      obe1
      (Vichas et al., 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)