Gr8aUAS.cLa, Gr98bUAS.cSa, Scer\GAL4Gr33a-GAL4 has abnormal taste perception phenotype
Gr66aUAS.cLa, Gr8aUAS.cLa, Gr98bUAS.cSa, Scer\GAL4Gr64f.9.7 has abnormal taste perception phenotype
Gr66aUAS.cLa, Gr8aUAS.cLa, Gr98bUAS.cSa, Scer\GAL4Ir76b.1.5 has abnormal taste perception phenotype
Co-expression of Gr8aScer\UAS.cLa and Gr98bScer\UAS.cSa in L-canavanine-insensitive S2- and I-type sensilla under the control of Scer\GAL4Gr33a-GAL4 confers robust L-canavanine responses to these sensilla.
Co-expression of Gr8aScer\UAS.cLa, Gr98bScer\UAS.cSa and Gr66aScer\UAS.cLa in sugar-activated gustatory receptor neurons in L-type sensilla under the control of Scer\GAL4Gr64f.9.7 confers robust L-canavanine responses to these sensilla. Expression of only two of the three constructs is insufficient to confer significant L-canavadine responsiveness, although in some cases, co-expression of Gr8aScer\UAS.cLa and Gr66aScer\UAS.cLa produces a small L-canavanine-evoked response in L-type sensilla.
Expressing Gr8aScer\UAS.cLa, Gr98bScer\UAS.cSa and Gr66aScer\UAS.cLa in low salt-sensing gustatory receptor neurons under the control of Scer\GAL4Ir76b.1.5 confers significant L-canavanine responsiveness to these neurons. They do not induce responsiveness to any bitter compounds.
Expression of Gr8aScer\UAS.cLa, Gr98bScer\UAS.cSa and Gr66aScer\UAS.cLa under the control of Scer\GAL4Gr64f.9.7 in sweet-sensing gustatory receptor neurons enhances the loss of preference for sucrose over sucrose laced with L-canavanine seen in Df(3L)ex83 mutant flies: the flies now exhibit a significant attraction to the L-canavanine-containing food. No enhancement is seen when any two of the three constructs is expressed.
Expression of Gr98bScer\UAS.cSa under the control of any of Scer\GAL4Gr66a.PD, Scer\GAL4Gr8a.0.7 or Scer\GAL4Gr98b.8.9 rescues the L-canavanine avoidance defects seen in Gr98b1 mutant flies. The loss of L-canavanine-induced action potentials is rescued when Gr98bScer\UAS.cSa is expressed under the control of Scer\GAL4Gr66a.PD.