FB2024_03 , released June 25, 2024
Allele: Scer\GAL4VT050660
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General Information
Symbol
Scer\GAL4VT050660
Species
S. cerevisiae
Name
FlyBase ID
FBal0310103
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{VT050660-GAL4}
Also Known As
VT50660-GAL4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Meadows, 2015.3.13)
Progenitor genotype
Carried in construct
P{VT050660-GAL4}
Cytology
Description

A 2108 base pair fragment from the flanking non-coding or intronic region of FeCH is fused upstream of a Drosophila synthetic core promoter (DSCP) followed by sequence encoding a GAL4 driver.

(Meadows, 2015.3.13)
Allele components
Component
Use(s)
Regulatory region(s)
VT050660
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult cerebrum | restricted
      (Bidaye et al., 2014)
      adult moonwalker ascending neuron
      (Sen et al., 2019, Bidaye et al., 2014)
      adult moonwalker descending neuron
      (Israel et al., 2022, Sen et al., 2019, Bidaye et al., 2014)
      adult neuron | subset of adult brain
      (Israel et al., 2022)
      Additional Information
      Statement
      Reference

      ScerGAL4VT050660 drives expression in moonwalker neurons as well as unidentified cells whose soma is located in the cerebrum.

      (Bidaye et al., 2014)
       
      Marker for
      adult moonwalker descending neuron
      (Israel et al., 2022)
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Thermogenetic activation (using TrpA1UAS.cvPa at 30[o]C) of Scer\GAL4VT050660 cells in adults results in a significant increase in the total distance and bout distance of backwards walking, with a significant decrease in forwards walking, compared to controls.

      Inhibition (using Ctet\TeTxLCTNT.UAS) of Scer\GAL4VT050660 cells significantly reduces backing-up behavior when adult flies reach a narrow dead end, compared to controls, this is also the case when inhibition is restricted to the adult stage using a Gal80[ts] transgene. Rather than walk backwards, these flies tend to stall for several minutes, before squeezing around to walk forwards in the opposite direction; there is a significant increase in total forwards walking distance in unimpeded conditions, compared to controls.

      (Bidaye et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4VT050660
      Name Synonyms
      Secondary FlyBase IDs
        References (5)