Imprecise excision of the progenitor P{EPgy2}Pol32EY15283 insertion, resulting in an approximately 14kb deletion that removes part of Pol32, the dao gene and part of ZnT35C.
~14 kb deletion resulting from the imprecise excision of the progenitor insertion. Genome coordinates of deletion reported by author.
female sterile (with PolD3R2)
male fertile (with PolD3R2)
suppressor of variegation (with PolD3R2), with In(1)wm4h
Pol32NR42/Pol32R2 females have short, thin bristles and moderate etching of the abdominal tergites. Mutant males have a more severe bristle and abdominal tergite phenotype.
Pol32NR42/Pol32R2 females lay eggs with an apparently normal chorion morphology, but they do not hatch. Embryos collected in a four hour period fall into three phenotypic classes: 11% have 1 nucleus, having a polar body with a star-like structure with additional or fragmented chromosomes, 57% arrest in mitotic cycles 1 to 6 with uneven distribution of nuclei and 31% appear to be degenerated, having no detectable chromatin.
Mitotic cells in Pol32NR42/Pol32R2 larval brains show a higher frequency of spontaneous chromosome breakage compared to controls. Three hours after treatment with 2.5 Gy X rays, more than 50% of mutant cells show chromosome breaks, a considerably higher proportion than control cells.
Pol32NR42/Pol32R2 animals are hypersensitive to ethyl methanesulphonate and to N-ethyl-N-nitrosourea compared to wild type.
Pol32NR42/Pol32R2 suppresses position effect variegation at the w locus caused by In(1)wm4h, but does not affect telomere position effect (TPE) in insertion lines carrying a w[+] marker.
PolD3R2/PolD3NR42 has embryo | maternal effect phenotype, non-suppressible | maternal effect by mei-W68k05603/mei-W68k05603
The types and frequency of defects seen in embryos derived from Pol32NR42/Pol32R2 females is not altered if the females are also homozygous for mei-W68k05603.