FB2024_03 , released June 25, 2024
Allele: Dmel\ERRRNAi.UAS.THFC
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General Information
Symbol
Dmel\ERRRNAi.UAS.THFC
Species
D. melanogaster
Name
FlyBase ID
FBal0302958
Feature type
allele
Associated gene
Dmel\ERR
Associated Insertion(s)
Carried in Construct
P{UAS-ERR.RNAi.THFC}
Key Links
Transgenic product class
RNAi_reagent
(Yu et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Yu et al., 2015)
Mutagen
in vitro construct
(Yu et al., 2015)
Progenitor genotype
Carried in construct
P{UAS-ERR.RNAi.THFC}
Cytology
Description

UAS regulatory sequences drive expression of an RNAi construct targeting ERR.

(Yu et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
ERR
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  azoospermia
      CEA
      (Yu et al., 2015)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Approximately 57% of Scer\GAL4c729>ERRdsRNA.Scer\UAS.THFC males are infertile.

      Approximately 45% of males expressing ERRdsRNA.Scer\UAS.THFC under the control of the cyst-cell specific driver Scer\GAL4C587 at 28[o]C.

      88% of the Scer\GAL4c729>ERRdsRNA.Scer\UAS.THFC males, and 77% of the Scer\GAL4C587>ERRdsRNA.Scer\UAS.THFC males have abnormal testis morphology, changing from the typical long tubular shape to a small and oval-shaped structure. Upon dissection, all germ cell stages are observed in most of the morphologically normal and many of the morphologically abnormal testes expressing ERRdsRNA.Scer\UAS.THFC under the control of either driver.

      Germ cells, hub cells and mature cyst cells are present in testes expressing ERRdsRNA.Scer\UAS.THFC. However, the number of pre-meiotic germ cells (gonioblasts, spermatogonia and spermatocytes) is strikingly reduced in Scer\GAL4c729>ERRdsRNA.Scer\UAS.THFC testes with morphological defects. Compared with wild-type, increased cell proliferation is detected in ERRdsRNA.Scer\UAS.THFC-expressing testes.

      (Yu et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      ERRRNAi.UAS.THFC
      ERRdsRNA.Scer\UAS.THFC
      ERRdsRNA.UAS.THFC
      Name Synonyms
      Secondary FlyBase IDs
        References (2)