FlyBase Allele Report: Dmel\Eato[MI14571]

General Information

Symbol

Dmel\Eato^MI14571

Species

D. melanogaster

Name

FlyBase ID

FBal0302332

Feature type

allele

Associated gene

Dmel\Eato

Associated Insertion(s)

Mi{MIC}Eato^MI14571

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

Mi{MIC}Eato^MI14571

Cytology

Description

Allele components

Component

Use(s)

Inserted element

Also carries

integrase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:13,784,705..13,784,705 [+]

(Bellen, 2015.2.9)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | adult stage | female | calorie restriction conditional (with Df(2L)BSC812)

(Santoso et al., 2018)

Phenotype Manifest In

egg chamber

(Santoso et al., 2018)

egg chamber (with Df(2L)BSC812)

(Santoso et al., 2018)

egg chamber (with Eato^f02254)

(Santoso et al., 2018)

egg chamber | calorie restriction conditional (with Df(2L)BSC812)

(Santoso et al., 2018)

follicle cell | calorie restriction conditional (with Df(2L)BSC812)

(Santoso et al., 2018)

nurse cell

(Santoso et al., 2018)

nurse cell (with Df(2L)BSC812)

(Santoso et al., 2018)

nurse cell (with Eato^f02254)

(Santoso et al., 2018)

nurse cell | calorie restriction conditional (with Df(2L)BSC812)

(Santoso et al., 2018)

Detailed Description

Statement

Reference

Eato^MI14571 homozygous as well as Eato^f02254/Eato^MI14571 mutant stage 14 egg chambers show nurse cell clearance defect and contain on average 6 persistent nurse cell nuclei, while in wild-type egg chambers all the germ-line derived nurse cells have undergone programmed cell death and been cleared by this stage. St.14 egg chambers containing Eato^MI14571 germ-line clones do not show significant clearance defects.

Similarly, Eato^MI14571/Df(2L)BSC812 mutants exhibit defects in engulfment of nurse cells by the follicle cells during starvation-induced programmed cell death: While phase 0 and phase 1 egg chambers resemble the control, in phase 3 eggs their follicle cells show fewer engulfed Dcp-1-positive vesicles and by phase 5 the dying mutant egg chambers display severe engulfment defects and follicle cell death, leaving behind completely unengulfed germline.

(Santoso et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Eato^MI14571 has egg chamber phenotype, suppressible | partially by Abca3^mir-1007-KO/Abca3^mir-1007-KO

(Santoso et al., 2018)

Eato^MI14571 has nurse cell phenotype, suppressible | partially by Abca3^mir-1007-KO/Abca3^mir-1007-KO

(Santoso et al., 2018)

Enhancer of

Statement

Reference

Eato^MI14571/Eato^MI14571 is an enhancer of egg chamber phenotype of Ced-12^HM05042, Scer\GAL4^GR1

(Santoso et al., 2018)

Eato^MI14571/Eato^MI14571 is an enhancer of nurse cell phenotype of Ced-12^HM05042, Scer\GAL4^GR1

(Santoso et al., 2018)

NOT Enhancer of

Statement

Reference

Eato^MI14571/Eato^MI14571 is a non-enhancer of egg chamber phenotype of drpr^Δ5

(Santoso et al., 2018)

Eato^MI14571/Eato^MI14571 is a non-enhancer of nurse cell phenotype of drpr^Δ5

(Santoso et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The nurse cell clearance defect observed in Eato^MI14571 mutant stage 14 egg chambers (which contain persistent nurse cell nuclei, while in wild-type egg chambers all the germ-line derived nurse cells have undergone programmed cell death and been cleared by this stage) is, surprisingly, ameliorated by ABCA^mir-1007-KO homozygosity, even though it strongly exacerbates this phenotype in Eato^f02254 mutant egg chambers.

The nurse cell clearance defects characteristic for drpr^Δ5 mutant stage 14 egg chambers are not significantly exacerbated by combination with Eato^MI14571 in homozygous state and both drpr^Δ5 single and Eato^MI14571;drpr^Δ5 double mutant egg chambers display on average ~9 persistent nuclei. However, Eato^MI14571 homozygosity does further impair the nurse cell engulfment defect seen in stage 14 egg chambers expressing Ced-12^HM05042 under the control of Scer\GAL4^GR1.

(Santoso et al., 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

59537

y¹ w^*; Mi{MIC}Eato^MI14571/SM6a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG31731^MI14571

(Santoso et al., 2018, Bellen, 2015.2.9)

Eato^MI14571

Eato^Mi

(Santoso et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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