FB2024_03 , released June 25, 2024
Allele: Dmel\rheaL334R.Ubi-p63E.EGFP
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General Information
Symbol
Dmel\rheaL334R.Ubi-p63E.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0301885
Feature type
allele
Associated gene
Dmel\rhea
Associated Insertion(s)
Carried in Construct
P{Ubi-talin.GFP.L334R}
Key Links
Transgenic product class
missense_variant
(Ellis et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Ellis et al., 2014)
Mutagen
in vitro construct
(Ellis et al., 2014)
Progenitor genotype
Carried in construct
P{Ubi-talin.GFP.L334R}
Cytology
Description

A Ubi-p63E promoter drives expression of a mutated form of rhea, which contains the amino acid replacement L334R (this mutation specifically abrogates the integrin-activating function of rhea without substantially affecting the ability of the rhea head to bind to integrin). The protein is tagged with EGFP.

(Ellis et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
Ubi-p63E
Encoded product / tool
rhea
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      rheaL334R.Ubi-p63E.EGFP partially rescues Df(3L)79a

      (Ellis et al., 2014)
      Comments

      Many embryos lacking maternal and zygotic rhea function (homozygous Df(3L)79a embryos derived from homozygous Df(3L)79a female germline clones) are able to hatch when rescued by maternally supplied rheaL334R.Ubi-p63E.T:Avic\GFP-EGFP. The rescued embryos do not have germband retraction or dorsal closure defects, but approximately 20% show muscle attachment defects at late stage 17.

      (Ellis et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      rheaL334R.Ubi-p63E.EGFP
      rheaL334R.Ubi-p63E.T:Avic\GFP-EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)