FB2024_03 , released June 25, 2024
Allele: Scer\GAL4opt.let7C.Δ3miR
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General Information
Symbol
Scer\GAL4opt.let7C.Δ3miR
Species
S. cerevisiae
Name
FlyBase ID
FBal0297446
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{let7CΔ3miR-GAL4}
Also Known As
let-7-CΔ3miR::optGal4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Wu et al., 2012)
Progenitor genotype
Carried in construct
P{let7CΔ3miR-GAL4}
Cytology
Description

A ~18kb fragment of genomic DNA containing a modified version of the lncRNA:let7C locus in which all three miRNA sequences (mir-100, let-7 and mir-125) are deleted is fused upstream of a Drosophila codon-optimized version of the GAL4::p65 driver (FBrf0212052). The resulting construct encodes a GAL4::p65 transcript that contains pri-lncRNA:let7C sequence in its 5'UTR.

(Wu et al., 2012)
Allele components
Component
Use(s)
Encoded product / tool
GAL4::p65
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      prepupal stage P4(ii) -- pupal stage P6
      neuron | subset of mushroom body

      Comment: 12-24h APF

      (Wu et al., 2012)
      Additional Information
      Statement
      Reference

      The number of neurons expressing ScerGAL4opt.let-7-C.Δ3miR increases during pupal development.

      (Wu et al., 2012)
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Scer\GAL4opt.let-7-C.Δ3miR
      Scer\GAL4opt.let7C.Δ3miR
      Name Synonyms
      Secondary FlyBase IDs
        References (6)