FlyBase Allele Report: Dmel\dpn[UAS.cWa]

General Information

Symbol

Dmel\dpn^UAS.cWa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Wallace

FlyBase ID

FBal0296582

Feature type

allele

Associated gene

Dmel\dpn

Associated Insertion(s)

Carried in Construct

P{UAS-dpn.W}

Also Known As

UAS-Dpn

Key Links

Transgenic product class

wild_type

(Wallace et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Wallace et al., 2000)

Mutagen

in vitro construct

(Wallace et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-dpn.W}

Cytology

Description

UASt regulatory sequences drive expression of the dpn open reading frame.

(Wallace et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dpn

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of brain cancer

CEA

(Huang et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of brain cancer

is ameliorated by eag^DN.EKI.UAS

(Huang et al., 2015)

is ameliorated by eag¹

(Huang et al., 2015)

is NOT ameliorated by Sh¹⁴

(Huang et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^GMR9D11

(Janssens et al., 2014)

abnormal neuroanatomy | adult stage, with Scer\GAL4^71B

(Wallace et al., 2000)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^71B

(Wallace et al., 2000)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Huang et al., 2015)

lethal - all die before end of larval stage | heat sensitive, with Scer\GAL4^insc-Mz1407

(Huang et al., 2015)

Phenotype Manifest In

adult brain, with Scer\GAL4^71B

(Wallace et al., 2000)

embryonic/larval optic lobe | third instar larval stage, with Scer\GAL4^71B

(Wallace et al., 2000)

type II neuroblast, with Scer\GAL4^GMR9D11

(Janssens et al., 2014)

type II neuroblast | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Huang et al., 2015)

type I neuroblast | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Huang et al., 2015)

Detailed Description

Statement

Reference

Expression of dpn^Scer\UAS.cWa under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[-] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}2) results in supernumerary type II neuroblasts in larval brains. A milder phenotype is seen when dpn^Scer\UAS.cWa is expressed in ase[+] immature INPs using P{GMR9D11-GAL4}attP2.

(Janssens et al., 2014)

Expression of dpn^Scer\UAS.cWa under the control of Scer\GAL4^71B results in a striking increase in the size of the brain lobes as compared to wild type brains. This phenotype is fully penetrant. There is an increase in the number of mitotically active cells across the entire surface of the enlarged brain. In addition, a breakdown of the mitotic domain pattern that is present in wild type third instar larval optic lobes is also evident.

(Wallace et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR9D11, dpn^UAS.cWa has abnormal neuroanatomy phenotype, enhanceable by erm^zf.VP16.UAS/Scer\GAL4^GMR9D11

(Janssens et al., 2014)

Suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, dpn^UAS.cWa has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by eag¹

(Huang et al., 2015)

Scer\GAL4^insc-Mz1407, dpn^UAS.cWa has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/eag^DN.EKI.UAS

(Huang et al., 2015)

Scer\GAL4^insc-Mz1407, dpn^UAS.cWa has lethal - all die before end of larval stage | heat sensitive phenotype, suppressible | partially by eag¹

(Huang et al., 2015)

Scer\GAL4^insc-Mz1407, dpn^UAS.cWa has lethal - all die before end of larval stage | heat sensitive phenotype, suppressible | partially by Scer\GAL4^insc-Mz1407/eag^DN.EKI.UAS

(Huang et al., 2015)

Scer\GAL4^GMR9D11, dpn^UAS.cWa has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^GMR9D11/erm^UAS.Tag:HA

(Janssens et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, dpn^UAS.cWa has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Sh¹⁴

(Huang et al., 2015)

Scer\GAL4^insc-Mz1407, dpn^UAS.cWa has lethal - all die before end of larval stage | heat sensitive phenotype, non-suppressible by Sh¹⁴

(Huang et al., 2015)

Enhancer of

Statement

Reference

dpn^UAS.cWa/Scer\GAL4^GMR9D11 is an enhancer of abnormal neuroanatomy phenotype of brat^DG19310/brat¹¹

(Janssens et al., 2014)

dpn^UAS.cWa/Scer\GAL4^GMR9D11 is an enhancer of abnormal neuroanatomy phenotype of Df(2L)erm²/erm¹, erm^Tag:FLAG

(Janssens et al., 2014)

Suppressor of

Statement

Reference

Scer\GAL4^da.PU/dpn^UAS.cWa is a suppressor of majority die during embryonic stage | maternal effect phenotype of sisRNA:4^MI00051

(Tay and Pek, 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR9D11, dpn^UAS.cWa has type II neuroblast phenotype, enhanceable by erm^zf.VP16.UAS/Scer\GAL4^GMR9D11

(Janssens et al., 2014)

Suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, dpn^UAS.cWa has type I neuroblast | third instar larval stage phenotype, suppressible by eag¹

(Huang et al., 2015)

Scer\GAL4^insc-Mz1407, dpn^UAS.cWa has type II neuroblast | third instar larval stage phenotype, suppressible by eag¹

(Huang et al., 2015)

Scer\GAL4^insc-Mz1407, dpn^UAS.cWa has type I neuroblast | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/eag^DN.EKI.UAS

(Huang et al., 2015)

Scer\GAL4^insc-Mz1407, dpn^UAS.cWa has type II neuroblast | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/eag^DN.EKI.UAS

(Huang et al., 2015)

Scer\GAL4^GMR9D11, dpn^UAS.cWa has type II neuroblast phenotype, suppressible by Scer\GAL4^GMR9D11/erm^UAS.Tag:HA

(Janssens et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, dpn^UAS.cWa has type I neuroblast | third instar larval stage phenotype, non-suppressible by Sh¹⁴

(Huang et al., 2015)

Scer\GAL4^insc-Mz1407, dpn^UAS.cWa has type II neuroblast | third instar larval stage phenotype, non-suppressible by Sh¹⁴

(Huang et al., 2015)

Enhancer of

Statement

Reference

dpn^UAS.cWa/Scer\GAL4^GMR9D11 is an enhancer of type II neuroblast | increased number phenotype of brat^DG19310/brat¹¹

(Janssens et al., 2014)

dpn^UAS.cWa/Scer\GAL4^GMR9D11 is an enhancer of type II neuroblast | increased number phenotype of Df(2L)erm²/erm¹, erm^Tag:FLAG

(Janssens et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of dpn^Scer\UAS.cWa under the control of Scer\GAL4^da.PU suppresses the lowered hatching rate phenotype seen in sisRNA:4^MI00051/sisRNA:4^MI00051 embryos.

(Tay and Pek, 2017)

eag¹ mutant third instar larvae display markedly reduces dpn^Scer\UAS.cWa-induced tumor growth.

Co-expression of eag^{DN.EKI.Scer\UAS} markedly reduces dpn^Scer\UAS.cWa-induced tumor growth.

(Huang et al., 2015)

Expression of dpn^Scer\UAS.cWa under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[-] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}2) enhances the supernumerary neuroblast phenotype seen in brat^DG19310/brat¹¹ mutant larval brains. A milder enhancement is seen when dpn^Scer\UAS.cWa is expressed using an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] INPs (P{GMR9D11-GAL4}attP2).

Expression of dpn^Scer\UAS.cWa under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2) enhances the supernumerary neuroblast phenotype seen in hypomorphic erm mutant larval brains (erm¹/Df(2L)erm² mutants expressing erm^T:Zzzz\FLAG).

Expression of erm^{Scer\UAS.T:Ivir\HA1} suppresses the supernumerary neuroblast phenotype seen in larval brains when dpn^Scer\UAS.cWa is expressed under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[-] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}2).

Expression of erm^{zf.Scer\UAS.T:Hsim\VP16} enhances the supernumerary neuroblast phenotype seen in larval brains when dpn^Scer\UAS.cWa is expressed under the control of an insertion of Scer\GAL4^GMR9D11 that expresses in ase[+] immature Intermediate Neural Progenitors (INPs) (P{GMR9D11-GAL4}attP2).

(Janssens et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dpn^Scer\UAS.cWa

dpn^UAS.cWa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Wallace

Secondary FlyBase IDs

References (6)

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