FlyBase Allele Report: Ecol\lexA[DBD.C1a.ort]

General Information

Symbol

Ecol\lexA^DBD.C1a.ort

Species

E. coli

Name

FlyBase ID

FBal0296550

Feature type

allele

Associated gene

Ecol\lexA

Associated Insertion(s)

Carried in Construct

P{ort-LexA.C1a.DBD}

Also Known As

ort^C1a-LexADBD

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Karuppudurai et al., 2014)

Progenitor genotype

Carried in construct

P{ort-LexA.C1a.DBD}

Cytology

Description

The ort C1 enhancer followed by the hs43 minimal promoter regulate expression of a lexA(DBD)::Zip- hemidriver (component of a 'split driver' system, contains the lexA DNA-binding domain).

(Karuppudurai et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

ort

Encoded product / tool

lexA(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

transmedullary neuron Tm20

Comment: when combined with P{dVP16AD}ET24a

(Melnattur et al., 2014)

transmedullary neuron Tm5c

Comment: when combined with P{dVP16AD}VGlut^{OK371-dVP16AD}

(Karuppudurai et al., 2014)

Additional Information

Statement

Reference

Marker for

transmedullary neuron Tm5a

(Karuppudurai et al., 2014)

transmedullary neuron Tm5b

(Karuppudurai et al., 2014)

transmedullary neuron Tm5c

(Karuppudurai et al., 2014)

Reflects expression of

ort

(Karuppudurai et al., 2014)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal phototaxis, with Ctet\tetX^{TNT-LC.13x.lexAop.Tag:HA}, Hsim\VP16^{AD-VGlut-OK371}

(Karuppudurai et al., 2014)

abnormal phototaxis, with Hsim\VP16^{AD-VGlut-OK371}, clumsy^JF02987

(Karuppudurai et al., 2014)

abnormal phototaxis, with Hsim\VP16^{AD-VGlut-OK371}, shi^{ts1.IVS.p10.20xUAS}

(Karuppudurai et al., 2014)

abnormal phototaxis, with Hsim\VP16^{AD-VGlut-OK371}, VGlut^JF02689

(Karuppudurai et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition (using Ctet\tetX^{TNT-LC.13x.lexAop.Tag:HA}) of neurons targeted by the split-lexA pair of Ecol\lexA^DBD.C1a.ort and Hsim\VP16^AD-ET24g in adults does not affect short-term aversive visual (blue/green) memory, compared to controls.

(Melnattur et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Ecol\lexA^DBD.C1a.ort, Hsim\VP16^{AD-VGlut-OK371}, clumsy^JF02987 has abnormal phototaxis phenotype, non-enhanceable by CG11155^JF03425, Ecol\lexA^DBD.C1a.ort, Hsim\VP16^{AD-VGlut-OK371}

(Karuppudurai et al., 2014)

Ecol\lexA^DBD.C1a.ort, Hsim\VP16^{AD-VGlut-OK371}, clumsy^JF02987 has abnormal phototaxis phenotype, non-enhanceable by Ecol\lexA^DBD.C1a.ort/Grik^JF01840, Hsim\VP16^{AD-VGlut-OK371}