A genomic region containing genomic lap and its full upstream and downstream untranslated regions has been tagged at the N-terminal end with the synthetic Flag-Tetracysteine (4C) peptide using recombineering. 4C is comprised of the Tag:FLAG peptide and a FlAsH binding tetracysteine tag (Tag:CALI(TC)).
Expression of lapN.4C.T:Zzzz\FLAG,T:Zzzz\TC almost completely rescues the reduction in excitatory junctional potentials (EJPs) amplitude seen in lap1/lapsd-3 mutants. The larval lethality is also rescued.