FlyBase Allele Report: Dmel\nAChRα4[rye]

General Information

Symbol

Dmel\nAChRα4^rye

Species

D. melanogaster

Name

nAChRα4^redeye

FlyBase ID

FBal0295291

Feature type

allele

Associated gene

Dmel\nAChRα4

Associated Insertion(s)

Carried in Construct

Also Known As

rye, rye^T227M

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Shi et al., 2014)

Mutagen

ethyl methanesulfonate

(Shi et al., 2014)

Progenitor genotype

Cytology

Description

Amino acid replacement: T227M.

(Shi et al., 2014)

Mutation of a conserved residue at the junction between the ligand bind domain and the trans-membrane domain.

(Shi et al., 2014)

Nucleotide substitution: C?T.

(Shi et al., 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:23,263,873..23,263,873 [+]

Nucleotide change:

C23263873T

Reported nucleotide change:

C?T

Amino acid change:

Reported amino acid change:

T227M

(Shi et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal sleep

(Shi et al., 2014)

abnormal sleep | dominant

(Shi et al., 2014)

decreased sleep | adult stage

(Vaccaro et al., 2020)

short lived

(Vaccaro et al., 2020)

Phenotype Manifest In

Detailed Description

Statement

Reference

nAChRα4^rye mutants show a severe reduction of sleep length: homozygotes show >50% reduction in both daytime and night-time sleep; heterozygotes have slightly less sleep than wildtype. The activity of homozygotes is comparable to controls, suggesting nAChRα4^rye is not hyperactive. The reduction in baseline sleep results largely from a shortening of the average sleep episode duration, indicating a defect in sleep maintenance - the average number of sleep episodes at night is actually increased significantly.

Most nAChRα4^rye homozygotes show normal circadian rhythms.

(Shi et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

nAChRα4^rye has abnormal sleep | dominant phenotype, enhanceable by qvr^+tKa

(Shi et al., 2014)

NOT Enhanced by

Statement

Reference

nAChRα4^rye has abnormal sleep | dominant phenotype, non-enhanceable by qvr[+]/qvr^EY04063

(Shi et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

nAChRα4^rye/+; qvr^EY04063/+ flies show sleep duration comparable to that of nAChRα4^rye/+ alone.

nAChRα4^rye/+; qvr^+tKa flies show a further reduction in sleep over that of nAChRα4^rye/+ alone.

(Shi et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

nAChRα4^rye is partially rescued by nAChRα4^UAS.cSa/Scer\GAL4^{nAChRα4.ryeP}

(Shi et al., 2014)

Comments

Scer\GAL4^{nAChRα4.ryeP}-mediated expression of nAChRα4^Scer\UAS.cSa partially rescues the nAChRα4^rye phenotype and restores total sleep to levels seen in nAChRα4^rye heterozygotes.

(Shi et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

80692

w¹¹¹⁸; nAChRα4^rye/TM6C, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

NAChRalpha4^rye

(Wen et al., 2024)

nAChRα4^rye

(Qiao et al., 2022, Öztürk-Çolak et al., 2020)

rye

(Axelrod et al., 2023, Lu et al., 2022, Shi et al., 2014)

rye^T227M

(Vaccaro et al., 2020, Shi et al., 2014)

α4^T227M

(Lu et al., 2022)

Name Synonyms

nAChRα4redeye

redeye

(Shi et al., 2014)

Secondary FlyBase IDs

References (7)

Report Sections

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