FB2024_03 , released June 25, 2024
Allele: Dmel\CkIIαUAS.N.Tag:HA
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General Information
Symbol
Dmel\CkIIαUAS.N.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0295270
Feature type
allele
Associated gene
Dmel\CkIIα
Associated Insertion(s)
Carried in Construct
P{UAS-HA-CkIIα}
Key Links
Transgenic product class
cDNA
(Bulat et al., 2014)
wild_type
(Bulat et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Bulat et al., 2014)
wild_type
(Bulat et al., 2014)
Mutagen
in vitro construct
(Bulat et al., 2014)
Progenitor genotype
Carried in construct
P{UAS-HA-CkIIα}
Cytology
Description

10xUAS regulatory sequences drive expression of CkIIα coding sequences (corresponding to LD27706) tagged with 3x Tag:HA sequences at the N-terminal end.

(Bulat et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
CkIIα
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of CkIIαScer\UAS.N.T:Ivir\HA1 under the control of Scer\GAL4da.G32 and Scer\GAL80elav.PS in a CkIIαP2 mutant background results in synaptic retractions at 43% of analyzed neuromuscular junctions.

      Expression of two copies of CkIIαScer\UAS.N.T:Ivir\HA1 under the control of two copies of Scer\GAL4elav-C155 results in a dramatic increase in the number of satellite boutons per neuromuscular junction. These altered synaptic boutons often contain only a single synapse, a feature never observed at control neuromuscular junctions.

      (Bulat et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Presynaptic expression of CkIIαScer\UAS.N.T:Ivir\HA1 in Ank2f02001 homozygous mutants significantly alleviates the synaptic retraction phenotype.

      (Bulat et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4elav-C155/CkIIαUAS.N.Tag:HA rescues CkIIαLL05896

      (Bulat et al., 2014)

      Scer\GAL4Mef2.PU/CkIIαUAS.N.Tag:HA rescues CkIIαLL05896

      (Bulat et al., 2014)
      Fails to rescue

      Scer\GAL4da.G32/CkIIαUAS.N.Tag:HA fails to rescue CkIIαLL05896

      (Bulat et al., 2014)

      Scer\GAL80elav.PS, Scer\GAL4da.G32, CkIIαUAS.N.Tag:HA fails to rescue CkIIαLL07221

      (Bulat et al., 2014)
      Comments

      Expression of CkIIαScer\UAS.N.T:Ivir\HA1 in the nervous system, under the control of Scer\GAL4elav-C155, rescues the synapse retraction phenotype seen in CkIIαP1 homozygous neuromuscular junctions.

      (Bulat et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CkIIαScer\UAS.N.T:Ivir\HA1
      CkIIαUAS.N.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (1)