Amino acid replacement: E859K.
UAS regulatory sequences drive expression of mutated drosha coding sequences containing a G to A change, creating a glutamic acid to lysine missense mutation in a conserved residue of the first RNase III domain, as well as a 441bp deletion, removing amino acids 561-707. This construct is tagged at the C-terminal with Tag:FLAG.
Expression of droshaΔ.E859K.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ey-OK107 in an otherwise wild-type background results in overgrowth of α/β neurons.
droshaΔ.E859K.UAS.Tag:FLAG/Scer\GAL4ey-OK107 fails to rescue drosha21K11