Amino acid replacement: E859K.
The droshaΔE859K allele contains a G to A change, creating a glutamic acid to lysine missense mutation in a conserved residue of the first RNase III domain, as well as a 441bp deletion, removing amino acids 561-707.
Inferred endpoints of 441 bp deletion that removes amino acids 561-707. Allele also carries a G to A base change causing a E to K amino acid substitution in an unspecified location in the first RNase III domain.
droshaΔE859K/Df(2R)Exel6055 transheterozygous animals all die at the larval-to-pupal transition. Approximately 10% die as late-stage larvae, whereas the remainder unsuccessfully attempt to pupariate. These animals exhibit a small central nervous system and lack imaginal discs.
Homozygous droshaΔE859K adult mushroom body clones display a reduction in cell number compared to controls, and the neurons present are predominantly early-born γ neurons. The γ lobes of the mutant mushroom clones are disorganised and extend across the midline.
droshaΔE859K single DL1 neuron clones innervate the DL1 glomerulus more sparsely and inappropriately targets additional glomeruli. While wild-type DL1 cell clones terminate their main branches at the lateral edge of the lateral horn, all droshaΔE859K single DL1 axons fail to reach the lateral horn lateral edge.
droshaΔE859K is rescued by Scer\GAL4ey-OK107/droshaUAS.Tag:FLAG
droshaΔE859K is rescued by droshaUAS.Tag:FLAG/Scer\GAL4GH146.PB
