FlyBase Allele Report: Dmel\tay[RNAi.UAS]

General Information

Symbol

Dmel\tay^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0294967

Feature type

allele

Associated gene

Dmel\tay

Associated Insertion(s)

Carried in Construct

P{UAS-tay.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Molnar and de Celis, 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Molnar and de Celis, 2013)

Mutagen

in vitro construct

(Molnar and de Celis, 2013)

Progenitor genotype

Carried in construct

P{UAS-tay.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of a 700bp inverted repeat targeting tay.

(Molnar and de Celis, 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

tay

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | adult stage, with Df(1)tay, Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

visible | adult stage, with Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

Phenotype Manifest In

wing, with Df(1)tay, Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

wing, with Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

wing vein | ectopic, with Df(1)tay, Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

wing vein | ectopic, with Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

Detailed Description

Statement

Reference

When tay^{dsRNA.Scer\UAS} is expressed in the wing blade under the control of Scer\GAL4^MD-638 the wings are reduced in size, display ectopic veins and show some defects in the most distal region of the wing margin. This phenotype is enhanced in a heterozygous Df(1)tay mutant background.

(Molnar and de Celis, 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^MD-638, tay^RNAi.UAS has visible | adult stage phenotype, enhanceable by rho^UAS.cdCa/Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

Scer\GAL4^MD-638, tay^RNAi.UAS has visible | adult stage phenotype, enhanceable by Scer\GAL4^MD-638/rl^UAS.Tag:HA

(Molnar and de Celis, 2013)

Scer\GAL4^MD-638, tay^RNAi.UAS has visible | adult stage phenotype, enhanceable by Mkp3^M76-R2b/Mkp3[+]

(Molnar and de Celis, 2013)

Scer\GAL4^MD-638, tay^RNAi.UAS has visible | adult stage phenotype, enhanceable by Mkp3^GD8219, Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

Enhancer of

Statement

Reference

tay^RNAi.UAS, Scer\GAL4^salm.EPv is an enhancer of visible | adult stage phenotype of Ras85D^V12.UAS, Scer\GAL4^salm.EPv

(Molnar and de Celis, 2013)

tay^RNAi.UAS/Scer\GAL4^MD-638 is an enhancer of visible | adult stage phenotype of Scer\GAL4^MD-638, rl^{Sem.UAS.Tag:HA}

(Molnar and de Celis, 2013)

tay^RNAi.UAS/Scer\GAL4^MD-638 is an enhancer of visible | adult stage phenotype of Scer\GAL4^MD-638, rho^UAS.cdCa

(Molnar and de Celis, 2013)

NOT Enhancer of

Statement

Reference

tay^RNAi.UAS, Scer\GAL4^MD-638 is a non-enhancer of visible | adult stage phenotype of Egfr^DN.UAS.cBa, Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^MD-638, tay^RNAi.UAS has wing vein | ectopic phenotype, enhanceable by rho^UAS.cdCa/Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

Scer\GAL4^MD-638, tay^RNAi.UAS has wing vein | ectopic phenotype, enhanceable by Scer\GAL4^MD-638/rl^UAS.Tag:HA

(Molnar and de Celis, 2013)

Scer\GAL4^MD-638, tay^RNAi.UAS has wing vein | ectopic phenotype, enhanceable by Mkp3^M76-R2b/Mkp3[+]

(Molnar and de Celis, 2013)

Scer\GAL4^MD-638, tay^RNAi.UAS has wing vein | ectopic phenotype, enhanceable by Mkp3^GD8219, Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

Enhancer of

Statement

Reference

tay^RNAi.UAS, Scer\GAL4^salm.EPv is an enhancer of wing vein | ectopic phenotype of Ras85D^V12.UAS, Scer\GAL4^salm.EPv

(Molnar and de Celis, 2013)

tay^RNAi.UAS/Scer\GAL4^MD-638 is an enhancer of wing vein | ectopic phenotype of Scer\GAL4^MD-638, rl^{Sem.UAS.Tag:HA}

(Molnar and de Celis, 2013)

tay^RNAi.UAS/Scer\GAL4^MD-638 is an enhancer of wing vein | ectopic phenotype of Scer\GAL4^MD-638, rho^UAS.cdCa

(Molnar and de Celis, 2013)

NOT Enhancer of

Statement

Reference

tay^RNAi.UAS, Scer\GAL4^MD-638 is a non-enhancer of wing vein phenotype of Egfr^DN.UAS.cBa, Scer\GAL4^MD-638

(Molnar and de Celis, 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of tay^{dsRNA.Scer\UAS} enhances the ectopic wing vein phenotype seen when Ras85D^V12.Scer\UAS is expressed under the control of Scer\GAL4^salm.EPv.

Co-expression of rho^{Scer\UAS.cdCa} and tay^{dsRNA.Scer\UAS} under the control of Scer\GAL4^MD-638 results in a stronger ectopic wing vein phenotype than when either transgene is expressed alone.

Expression of tay^{dsRNA.Scer\UAS} does not modify the wing vein loss phenotype seen when Egfr^{DN.Scer\UAS.cBa} is expressed under the control of Scer\GAL4^MD-638.

Expression of rl^{Scer\UAS.T:Ivir\HA1} slightly enhances the ectopic wing vein phenotype seen when tay^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^MD-638.

Co-expression of rl^{Sem.Scer\UAS.T:Ivir\HA1} and tay^{dsRNA.Scer\UAS} under the control of Scer\GAL4^MD-638 results in a stronger ectopic wing vein phenotype than when either transgene is expressed alone.

One copy of Mkp3^M76-R2b enhances the wing vein phenotype seen when tay^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^MD-638.

Expression of Mkp3^GD8219 enhances the wing vein phenotype seen when tay^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^MD-638.

(Molnar and de Celis, 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

tay^RNAi.UAS/Scer\GAL4^salm.EPv rescues tay^EP-866

(Molnar and de Celis, 2013)

tay^RNAi.UAS/Scer\GAL4^dpp.3KK rescues tay^EP-866

(Molnar and de Celis, 2013)

Comments

Expression of tay^{dsRNA.Scer\UAS} suppresses the wing size reduction and loss of veins observed when tay^EP-866 is expressed under the control of either Scer\GAL4^salm.EPv or Scer\GAL4^dpp.3KK.

(Molnar and de Celis, 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

tay^RNAi.UAS

tay^{dsRNA.Scer\UAS}

tay^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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