FB2024_03 , released June 25, 2024
Allele: Dmel\Gem2UAS.cBa
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General Information
Symbol
Dmel\Gem2UAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct of Borg
FlyBase ID
FBal0294749
Feature type
allele
Associated gene
Dmel\Gem2
Associated Insertion(s)
Carried in Construct
P{UAS-Gem2.B}
Also Known As
UAS.Gem2FL
Key Links
Transgenic product class
wild_type
(Borg and Cauchi, 2013)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Borg and Cauchi, 2013)
Mutagen
in vitro construct
(Borg and Cauchi, 2013)
Progenitor genotype
Carried in construct
P{UAS-Gem2.B}
Cytology
Description

UASt regulatory sequences drive expression of the full length Gem2 coding sequence.

(Borg and Cauchi, 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Gem2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Gem2Scer\UAS.cBa under the control of Scer\GAL4Mef2.PR has no effect on flight ability of adult flies, mobility of the third instar larvae or shape of pupae and Scer\GAL4elav-C155-driven expression does not impair adult viability.

      (Lanfranco et al., 2017)

      Expression of Gem2Scer\UAS.cBa driven by Scer\GAL4Mef2.PR impairs flight in early adulthood and flies have either droopy or held up wings, compared to controls; survival does not decline with age. Expression of Gem2Scer\UAS.cBa driven by Scer\GAL4how-24B results in pupae with a significantly larger puparial axial ratio than controls.

      Expression of a low expressing in combination with a high expressing Gem2Scer\UAS.cBa driven by Scer\GAL41032.hx has a marginal impact on viability to adulthood (around 80% survive); expression of two high expressing Gem2Scer\UAS.cBa transgenes results in almost total lethality at 25[o]C (few escapers), and complete lethality at 29[o]C.

      (Borg et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Simultaneous expression of Gem3ΔN.Scer\UAS and Gem2Scer\UAS.cBa under the control of Scer\GAL4Mef2.PR leads to strongly reduced mobility in third instar larvae and more elongated shape of the puparium.

      (Lanfranco et al., 2017)

      Co-expression of Gem3ΔN.Scer\UAS and Gem2Scer\UAS.cBa driven by Scer\GAL4Mef2.PR results in lethality.

      (Borg et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4da.PU/Gem2UAS.cBa rescues Gem2NIG.10419R

      (Borg and Cauchi, 2013)
      Comments

      Expression of Gem2Scer\UAS.cBa suppresses the lethality seen when Gem2NIG.10419R is expressed under the control of Scer\GAL4da.PU.

      (Borg and Cauchi, 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Gem2Scer\UAS.cBa
      Gem2UAS.cBa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct of Borg
      Secondary FlyBase IDs
        References (3)