FB2024_03 , released June 25, 2024
Allele: Dmel\Gem3UAS.cCa
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General Information
Symbol
Dmel\Gem3UAS.cCa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct of Cauchi
FlyBase ID
FBal0294202
Feature type
allele
Associated gene
Dmel\Gem3
Associated Insertion(s)
Carried in Construct
P{UAS-Gem3.C}
Key Links
Transgenic product class
cDNA
(Cauchi et al., 2008)
wild_type
(Cauchi et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Cauchi et al., 2008)
wild_type
(Cauchi et al., 2008)
Mutagen
in vitro construct
(Cauchi et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-Gem3.C}
Cytology
Description

UASt regulatory sequences drive expression of a Gem3 cDNA.

(Cauchi et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Gem3
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Gem3Scer\UAS.cCa under the control of Scer\GAL4Mef2.PR has no effect on viability or flight ability.

      Flies co-expressing Gem3Scer\UAS.cCa, Gem3GD17221 and Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR are flight defective.

      (Borg and Cauchi, 2013)

      Expression of Gem3Scer\UAS.cCa under the control of Scer\GAL41032.hx results in a significant decrease in normalised neuromuscular junction (NMJ) area and a significant decrease in branch numbers per NMJ.

      (Cauchi et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4Mef2.PR/Gem3UAS.cCa rescues Gem3GD17221

      (Borg and Cauchi, 2013)

      Scer\GAL41032.hx/Gem3UAS.cCa rescues Gem3GD17221

      (Borg and Cauchi, 2013)

      Scer\GAL4Mef2.PR/Gem3UAS.cCa rescues Gem3ΔN.UAS

      (Borg and Cauchi, 2013)

      Scer\GAL4c179/Gem3UAS.cCa rescues Gem3GD17221

      (Cauchi et al., 2008)

      Scer\GAL4how-24B/Gem3UAS.cCa rescues Gem3GD17221

      (Cauchi et al., 2008)

      Scer\GAL41032.hx/Gem3UAS.cCa rescues Gem3rL562/Gem3e03688

      (Cauchi et al., 2008)

      Scer\GAL41032.hx/Gem3UAS.cCa rescues Gem3rL562

      (Cauchi et al., 2008)

      Scer\GAL4Act5C.PU/Gem3UAS.cCa rescues Gem3ΔN.UAS

      (Cauchi et al., 2008)

      Scer\GAL4c179/Gem3UAS.cCa rescues Gem3ΔN.UAS

      (Cauchi et al., 2008)

      Scer\GAL4how-24B/Gem3UAS.cCa rescues Gem3ΔN.UAS

      (Cauchi et al., 2008)

      Gem3UAS.cCa/Scer\GAL4G7 rescues Gem3ΔN.UAS

      (Cauchi et al., 2008)

      Scer\GAL4Mef2.PR/Gem3UAS.cCa rescues Gem3ΔN.UAS

      (Cauchi et al., 2008)

      Gem3UAS.cCa/Scer\GAL4C57 rescues Gem3ΔN.UAS

      (Cauchi et al., 2008)

      Scer\GAL4Act5C.PU/Gem3UAS.cCa rescues Gem3GD17221

      (Cauchi et al., 2008)
      Partially rescues

      Scer\GAL4Mef2.PR, Gem3UAS.cCa, Gem3GD17221 partially rescues Gem3ΔN.UAS

      (Borg and Cauchi, 2013)
      Fails to rescue

      Scer\GAL41032.hx/Gem3UAS.cCa fails to rescue Gem3e03688

      (Cauchi et al., 2008)
      Comments

      Expression of Gem3Scer\UAS.cCa rescues the flight defects seen in flies expressing Gem3GD17221 under the control of Scer\GAL4Mef2.PR.

      Expression of Gem3Scer\UAS.cCa rescues the flight defects seen in flies expressing Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR.

      Expression of Gem3Scer\UAS.cCa rescues the lethality seen in flies co-expressing Gem3GD17221 and Gem3ΔN.Scer\UAS under the control of Scer\GAL4Mef2.PR. The rescued flies exhibit flight defects.

      Expression of Gem3Scer\UAS.cCa rescues the lethality seen when Gem3GD17221 is expressed under the control of Scer\GAL41032.hx.

      (Borg and Cauchi, 2013)

      The lethality caused by expression of Gem3ΔN.Scer\UAS under the control of one of Scer\GAL4Act5C.PU, Scer\GAL4c179, Scer\GAL4how-24B, Scer\GAL4G7, Scer\GAL4Mef2.PR or Scer\GAL4C57 is significantly suppressed by co-expression of Gem3Scer\UAS.cCa.

      Expression of Gem3Scer\UAS.cCa rescues the lethality seen when Gem3GD17221 is expressed under the control of one of Scer\GAL4Act5C.PU, Scer\GAL4c179 or Scer\GAL4how-24B.

      (Cauchi et al., 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Gem3Scer\UAS.cCa
      Gem3UAS.cCa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct of Cauchi
      Secondary FlyBase IDs
        References (2)