FlyBase Allele Report: Dmel\Nos[UAS.cUa]

General Information

Symbol

Dmel\Nos^UAS.cUa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Unknown

FlyBase ID

FBal0294069

Feature type

allele

Associated gene

Dmel\Nos

Associated Insertion(s)

Carried in Construct

P{UAS-Nos.U}

Also Known As

UAS-NOS

Key Links

Transgenic product class

wild_type

(Taghli-Lamallem et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Taghli-Lamallem et al., 2014)

Mutagen

in vitro construct

(Taghli-Lamallem et al., 2014)

Progenitor genotype

Carried in construct

P{UAS-Nos.U}

Cytology

Description

UAS regulatory sequences drive expression of Nos.

(Taghli-Lamallem et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Nos

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Alexander disease

modeled by Hsap\GFAP^R79H.UAS

(Wang et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | third instar larval stage, with Scer\GAL4^phtm.PO

(Jaszczak et al., 2016)

Phenotype Manifest In

eye disc | third instar larval stage, with Scer\GAL4^phtm.PO

(Jaszczak et al., 2016)

Detailed Description

Statement

Reference

Ectopic expression of Nos^Scer\UAS.cUa under the control of Scer\GAL4^phm.PO and in the absence of any tissue damage inhibits eye disc growth in third instar larvae compared to age-matched controls.

(Jaszczak et al., 2016)

Over-expression of Nos^Scer\UAS.cUa driven in glia by Scer\GAL4^repo.PU does not produce toxicity.

(Wang et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Nos^UAS.cUa, Scer\GAL4^phtm.PO has abnormal size | third instar larval stage phenotype, non-suppressible by Lgr3^GL01056, Scer\GAL4^phtm.PO

(Jaszczak et al., 2016)

Enhancer of

Statement

Reference

Nos^UAS.cUa, Scer\GAL4^repo.PU is an enhancer of increased cell death | adult stage phenotype of Hsap\GFAP^R79H.UAS, Scer\GAL4^repo.PU

(Wang et al., 2015)

Nos^UAS.cUa, Scer\GAL4^repo.PU is an enhancer of bang sensitive phenotype of Hsap\GFAP^R79H.UAS, Scer\GAL4^repo.PU

(Wang et al., 2015)

Nos^UAS.cUa, Scer\GAL4^repo.PU is an enhancer of increased cell death | adult stage | cell non-autonomous phenotype of Hsap\GFAP^R79H.UAS, Scer\GAL4^repo.PU

(Wang et al., 2015)

NOT Enhancer of

Statement

Reference

Nos^UAS.cUa, Scer\GAL4^repo.PU is a non-enhancer of abnormal neuroanatomy | adult stage phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^repo.PU

(Wang et al., 2015)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Lgr3^GL01056, Nos^UAS.cUa has eye disc | third instar larval stage phenotype, non-suppressible by Lgr3^GL01056/Scer\GAL4^phtm.PO

(Jaszczak et al., 2016)

Enhancer of

Statement

Reference

Nos^UAS.cUa, Scer\GAL4^repo.PU is an enhancer of adult brain phenotype of Hsap\GFAP^R79H.UAS, Scer\GAL4^repo.PU

(Wang et al., 2015)

Nos^UAS.cUa, Scer\GAL4^repo.PU is an enhancer of neuron | adult stage | cell non-autonomous phenotype of Hsap\GFAP^R79H.UAS, Scer\GAL4^repo.PU

(Wang et al., 2015)

NOT Enhancer of

Statement

Reference

Nos^UAS.cUa, Scer\GAL4^repo.PU is a non-enhancer of adult brain phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^repo.PU

(Wang et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The eye disc growth inhibition (in the absence of tissue damage) observed in third instar larvae expressing Nos^Scer\UAS.cUa under the control of Scer\GAL4^phm.PO cannot be suppressed by co-expression of Lgr3^GL01056 (together with UAS-Dicer2 to enhance RNAi efficiency).

(Jaszczak et al., 2016)

Xenogenetic Interactions

Statement

Reference

Co-expression of Nos^Scer\UAS.cUa significantly enhances cell death (in both glia and non-autonomously in neurons) and seizure frequency in flies with expression of Hsap\GFAP^{R79H.Scer\UAS} driven by Scer\GAL4^repo.PU. Co-expression of Nos^Scer\UAS.cUa does not significantly alter neurodegeneration in flies with expression of Hsap\ATXN3^{tr.Q78.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^repo.PU.

(Wang et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Nos^Scer\UAS.cUa

Nos^UAS.cUa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Unknown

Secondary FlyBase IDs

References (3)

Report Sections

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