FB2024_03 , released June 25, 2024
Allele: Dmel\α-Cat::shgshg.ΔCyt.UASp
Open Close
General Information
Symbol
Dmel\α-Cat::shgshg.ΔCyt.UASp
Species
D. melanogaster
Name
FlyBase ID
FBal0291803
Feature type
allele
Associated gene
α-Cat::shg
Associated Insertion(s)
Carried in Construct
P{UASp-α-Cat::shg.shg.ΔCyt}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Pacquelet and Rorth, 2005)
Progenitor genotype
Carried in construct
P{UASp-α-Cat::shg.shg.ΔCyt}
Cytology
Description

Full-length α-Cat is fused to shg directly after the transmembrane domain of shg (at bp 4746). The cytoplasmic domain of shg is absent. The construct is cloned downstream of UASp regulatory sequences.

(Pacquelet and Rorth, 2005)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
α-Cat::shg
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of shg::α-Catshg.ΔCyt.Scer\UAS under the control of Scer\GAL4Scer\FRT.Rnor\CD2.αTub84B gives rise to very mild migration defects.

      (Pacquelet and Rorth, 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The co-expression of α-Cat::shgshg.ΔCyt.UASp does not suppress the ionizing radiation-induced basal cell delamination in the wing disc posterior compartment observed in individuals expressing BacA\p35UAS.cHa under the control of Scer\GAL4en.PU.

      (Dekanty et al., 2015)

      Expression of shg::α-Catshg.ΔCyt.Scer\UAS in follicle cells using the MARCM system and Scer\GAL4Scer\FRT.Rnor\CD2.αTub84B fails to suppress the follicle cell sorting, loss of epithelial integrity and oocyte mispositioning phenotypes seen in shgR69/+ mutant follicle cell clones.

      Expression of shg::α-Catshg.ΔCyt.Scer\UAS in wild-type egg chambers using the MARCM system and Scer\GAL4Scer\FRT.Rnor\CD2.αTub84B leads to a very mild cell migration defect. In mixed border cell clusters, in which half of the cells are wild-type and the other half are shgR69 mutant cells expressing shg::α-Catshg.ΔCyt.Scer\UAS, mild delays in migration are seen. This is similar to the phenotype seen in border cells that are half wild-type and half shgR69 mutant. Interestingly, in the presence of shg::α-Catshg.ΔCyt.Scer\UAS, mixed shgR69/wild-type border cell clusters are never split, and all possible ratios of wild-type to mutant cells are observed.

      (Pacquelet and Rorth, 2005)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      shg::α-Catshg.ΔCyt.Scer\UAS.P\T
      shg::α-Catshg.ΔCyt.Scer\UAS
      α-Cat::shgshg.ΔCyt.Scer\UAS.P\T
      α-Cat::shgshg.ΔCyt.UASp
      Name Synonyms
      Secondary FlyBase IDs
        References (3)