Imprecise excision of P{EP}SyndEP877, resulting in a 536bp deletion extending to the right of the original insertion site. This removes approximately half of the first exon and part of the first intron of Synd.
A 536 bp deletion resulting from the imprecise excision of P{EP}SyndEP877, extends to the right from the insertion site.
Pupal ommatidia of Syndmut1/Synd661T and Syndmut1/Df(3R)BSC43 transheterozygotes, but not Syndmut1 homozygotes, exhibit C-shaped peripheral photoreceptor rhabdomeres when compared to controls.
Almost all homozygous and hemizygous Syndmut1 animals die at the third instar larval stage.
In Syndmut1/Df(3R)BSC43 flies, the proportion of multi-nucleated onion-stage spermatids increases by almost 20-fold compared to controls.
Syndmut1/Df(3R)BSC43 spermatocytes display a poorly organised central spindle and a fragmented contractile ring. Time-lapse imaging shows that the peripheral microtubules of mutants are less robust than controls, and the central spindle fails to form in an organised manner after anaphase onset. Unstable peripheral microtubule bundles occasionally form but then disintegrate with failure of cleavage furrow ingression. Spindles in Syndmut1/Df(3R)BSC43 spermatocytes are often mispositioned with a gap between the centrosomes and the cortex. Ingression of the cleavage furrow in the mutants is often asymmetric, failing to progress to the interior of the cell and then regressing. These abnormal microtubule dynamics are observed in 64% of mutant spermatocytes, compared to 0% in controls.
