FB2024_03 , released June 25, 2024
Allele: Dmel\PvrCA.UAS
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General Information
Symbol
Dmel\PvrCA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0291422
Feature type
allele
Associated gene
Dmel\Pvr
Associated Insertion(s)
Carried in Construct
P{UAS-Pvr.CA}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Bond and Foley, 2012)
Progenitor genotype
Carried in construct
P{UAS-Pvr.CA}
Cytology
Description

UAS regulatory sequences drive expression of constitutively active Pvr coding sequences.

(Bond and Foley, 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Pvr
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of PvrCA.Scer\UAS under the control of Scer\GAL4crq.PO does not disrupt embryonic hemocyte migration.

      (Parsons and Foley, 2013)

      Expression of PvrCA.Scer\UAS specifically for 10 days 3-5 days after eclosion, under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B results in a striking expansion of esg>GFP positive cell clusters with a distinctive cellular morphology indicating cell proliferation.

      Approximately half of Scer\GAL4esg-NP5130 --> PvrCA.Scer\UAS flies succumb to infection with P. entomophila within 64 hours of infection, as in wild-type.

      (Bond and Foley, 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      PvrCA.UAS, Scer\GAL4NP5130 has adult midgut phenotype, suppressible by Ras85DN17.UAS, Scer\GAL4NP5130

      (Bond and Foley, 2012)
      NOT suppressed by
      Statement
      Reference

      PvrCA.UAS, Scer\GAL4NP5130 has adult midgut phenotype, non-suppressible by bskK53R.UAS, Scer\GAL4NP5130

      (Bond and Foley, 2012)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of PvrCA.Scer\UAS under the control of Scer\GAL4crq.PO partially rescues the embryonic lethality of Df(2L)Pvf2-3/Df(2L)Pvf2-3 mutants and fully restore the defects in posterior migration of ventrally located hemocytes but does not restore the disrupted passage of hemocytes across the ventral nerve cord or their failure to invade the extended germband of Df(2L)Pvf2-3 mutant embryos.

      (Parsons and Foley, 2013)

      Co-expression of PvrCA.Scer\UAS and bskK53R.Scer\UAS in 3-5 day old adult flies, under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B phenocopies the proliferation of Scer\GAL4esg-NP5130>GFP-positive cells observed upon expression of PvrCA.Scer\UAS alone.

      Co-expression of Ras85DN17.Scer\UAS and PvrCA.Scer\UAS under the control of Scer\GAL4esg-NP5130 and Scer\GAL80ts.αTub84B significantly abrogates the PvrCA.Scer\UAS dysplastic phenotype, indicating that Ras85D is a downstream signaling component in the Pvr-dependent regulation of intestinal homeostasis.

      (Bond and Foley, 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4crq.PO/PvrCA.UAS partially rescues Pvr5363

      (Parsons and Foley, 2013)
      Comments

      Expression of PvrCA.Scer\UAS under the control of Scer\GAL4crq.PO in the Pvr5363 homozygous mutant background restores the impaired posterior migration of ventral hemocytes and partially rescues the failed transition of hemocytes across the ventral nerve cord in the anterior (but not posterior) of the mutant embryos. It however does not restore the invasion of hemocytes into the extended germband.

      (Parsons and Foley, 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      PvrCA.Scer\UAS
      PvrCA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)