FB2024_03 , released June 25, 2024
Allele: Dmel\eyaK699Q.UAS.Tag:Myr(v-Src)
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General Information
Symbol
Dmel\eyaK699Q.UAS.Tag:Myr(v-Src)
Species
D. melanogaster
Name
FlyBase ID
FBal0287992
Feature type
allele
Associated gene
Dmel\eya
Associated Insertion(s)
Carried in Construct
P{UAS-eya.K699Q.Myr}
Key Links
Transgenic product class
missense_variant
(Xiong et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Xiong et al., 2009)
Mutagen
in vitro construct
(Xiong et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-eya.K699Q.Myr}
Cytology
Description

UAS regulates expression of eya carrying the K699Q missense mutation (that ablates in vitro phosphatase activity, retains productive interactions with so, and exhibits reduced activity in both ectopic eye and genetic rescue assays) with the Tag:Myr(v-Src) myristoylation sequence inserted at the N-terminus.

(Xiong et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
eya
Tagged with
Tag:Myr(v-Src)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Scer\GAL4dpp.blk1-mediated expression of eyaK699Q.Scer\UAS.T:Uuuu\Myr4 does not induce ectopic eyes.

      Co-expression of eyaWT.Scer\UAS.T:SV40\nls2 and eyaK699Q.Scer\UAS.T:Uuuu\Myr4 via Scer\GAL4dpp.blk1 results in a limited increase (~10%) in ectopic eye induction relative to expression of eyaWT.Scer\UAS.T:SV40\nls2 alone.

      Scer\GAL4GMR.PU eyaK699Q.Scer\UAS.T:Uuuu\Myr4 eye/brain complexes show an average of 11.8 overshooting axon bundles per brain, compared to 8.4 in controls.

      (Xiong et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      eyaWT.UAS.Tag:NLS(SV40-largeT), Scer\GAL4dpp.blk1, eyaK699Q.UAS.Tag:Myr(v-Src) partially rescues eya2

      (Xiong et al., 2009)
      Comments

      Scer\GAL4dpp.blk1-mediated expression of eyaK699Q.Scer\UAS.T:Uuuu\Myr4 does not rescue the eyeless phenotype of eya2 animals.

      Co-expression of eyaWT.Scer\UAS.T:SV40\nls2 and eyaK699Q.Scer\UAS.T:Uuuu\Myr4 via Scer\GAL4dpp.blk1 results in a greater rescue of the eyeless phenotype of eya2 animals relative to expression of eyaWT.Scer\UAS.T:SV40\nls2 alone, though not as great a rescue as when eyaWT.Scer\UAS.T:Uuuu\Myr4 is co-expressed.

      (Xiong et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      eyaK699Q.Scer\UAS.T:Uuuu\Myr-v-Src
      eyaK699Q.Scer\UAS.T:Uuuu\Myr4
      eyaK699Q.UAS.Tag:Myr(v-Src)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)