FB2024_03 , released June 25, 2024
Allele: Scer\GAL4NPF.PU
Open Close
General Information
Symbol
Scer\GAL4NPF.PU
Species
S. cerevisiae
Name
neuropeptide F promoter construct of Unknown
FlyBase ID
FBal0287827
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{NPF-GAL4.U}
Also Known As
npf-Gal4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Johard et al., 2008)
Progenitor genotype
Carried in construct
P{NPF-GAL4.U}
Cytology
Description

NPF regulatory sequences control expression of a GAL4 driver.

(Johard et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
NPF
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      embryonic/larval brain | restricted
      (Johard et al., 2008)
      adult stage
      adult brain | restricted
      (Johard et al., 2008)
      Additional Information
      Statement
      Reference

      ScerGAL4NPF.PU drives expression in neurons that arborise in the dorsal brain, close to the alpha lobes and the peduncle. In adults, co-localisation between ScerGAL4NPF.PU and sNPF protein is found in one pair of protocerebral neurons. No co-localisation was found in the larval brain.

      (Johard et al., 2008)
       
      Marker for
      Reflects expression of
      NPF
      (Johard et al., 2008)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Thermogenetic activation (using TrpA1UAS.(B).cKa at 31[o]C) of Scer\GAL4NPF.PU cells in larvae significantly increases mouth hook contractions, compared to controls.

      (Pu et al., 2018)

      When males flies in which the Scer\GAL4NPF.PU-expressing neurons have been silenced are exposed to other males that have been engineered to produce female pheromones (by expressing traScer\UAS.cFa under the control of Scer\GAL4OK72) they do not show the increase in starvation resistance compared to females that is seen when they are exposed to wild type males.

      Activation of Scer\GAL4ppk23.2.695-expressing neurons in wild type males results in reduced lifespan compared with wild type males.

      (Gendron et al., 2014)

      Ablation (using rprUAS.cUa) of Scer\GAL4NPF.PU cells in adult males does not affect food intake, compared to controls.

      (Al-Anzi et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Statement
      Reference

      Scer\GAL4NPF.PU, TrpA1UAS.(B).cKa has abnormal feeding behavior | larval stage | heat sensitive phenotype, non-enhanceable by Dop1R1KK102341, Scer\GAL4NPF.PU

      (Pu et al., 2018)
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4NPF.PU, TrpA1UAS.(B).cKa has abnormal feeding behavior | larval stage | heat sensitive phenotype, non-suppressible by Dop1R1KK102341, Scer\GAL4NPF.PU

      (Pu et al., 2018)
      NOT Enhancer of
      Statement
      Reference

      Dop1R1KK102341, Scer\GAL4NPF.PU is a non-enhancer of abnormal feeding behavior | larval stage | heat sensitive phenotype of Scer\GAL4NPF.PU, TrpA1UAS.(B).cKa

      (Pu et al., 2018)
      NOT Suppressor of
      Statement
      Reference

      Dop1R1KK102341, Scer\GAL4NPF.PU is a non-suppressor of abnormal feeding behavior | larval stage | heat sensitive phenotype of Scer\GAL4NPF.PU, TrpA1UAS.(B).cKa

      (Pu et al., 2018)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Scer\GAL4NPF.PU
      Name Synonyms
      neuropeptide F promoter construct of Unknown
      Secondary FlyBase IDs
        References (14)