βTub85D promoter sequences drive expression of Ack in which a putative clathrin-binding box has been mutated (LID starting at residue 549 is mutated to AAA). The protein is tagged at the N-terminal end with mCherry.
AckCB.βTub85D.mCherry rescues Ack86
AckCB.βTub85D.T:Disc\RFP-mCherry restores fertility and sperm formation in Ack86 males.