βTub85D promoter sequences drive expression of Ack lacking amino acid residues 1-90 (removes the N-terminal SAM motif). The protein is tagged at the N-terminal end with mCherry.
AckΔS.βTub85D.mCherry fails to rescue Ack86
AckΔS.βTub85D.T:Disc\RFP-mCherry fails to rescue the sterility of Ack86 males.