FB2024_03 , released June 25, 2024
Allele: Dmel\CaMKIIT306A.T307A.UAS
Open Close
General Information
Symbol
Dmel\CaMKIIT306A.T307A.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0285584
Feature type
allele
Associated gene
Dmel\CaMKII
Associated Insertion(s)
Carried in Construct
P{UAS-CaMKII.T306A.T307A}
Key Links
Transgenic product class
missense_variant
(Malik et al., 2013)
polypeptide_post_translational_processing_variant
(Malik et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Malik et al., 2013)
polypeptide_post_translational_processing_variant
(Malik et al., 2013)
Mutagen
in vitro construct
(Malik et al., 2013)
Progenitor genotype
Carried in construct
P{UAS-CaMKII.T306A.T307A}
Cytology
Description

UAS regulatory sequences drive expression of CaMKII in which inhibitory phosphorylation sites have been mutated to blocking residues (amino acid replacements T306A and T307A).

(Malik et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
CaMKII
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing CaMKIIT306A.T307A.Scer\UAS under the control of either Scer\GAL4ey-OK107, Scer\GAL4c305a, Scer\GAL4Mef2.247 or Scer\GAL4CaMKII.7 show normal learning (measured as 2 minute memory) in an olfactory aversive conditioning assay.

      Flies expressing CaMKIIT306A.T307A.Scer\UAS under the control of either Scer\GAL4ey-OK107 or Scer\GAL4c305a show severely reduced middle-term memory (measured 3 hours after one cycle of training) in an olfactory aversive conditioning assay.

      Flies expressing CaMKIIT306A.T307A.Scer\UAS under the control of Scer\GAL4Mef2.247 show normal middle-term memory (measured 3 hours after one cycle of training) in an olfactory aversive conditioning assay.

      Flies expressing CaMKIIT306A.T307A.Scer\UAS under the control of either Scer\GAL4ey-OK107 or Scer\GAL4c305a show severely reduced long-term memory (measured 24 hours after 5 cycles of spaced training) in an olfactory aversive conditioning assay.

      Flies expressing CaMKIIT306A.T307A.Scer\UAS under the control of Scer\GAL4Mef2.247 show normal long-term memory (measured 24 hours after 5 cycles of spaced training) in an olfactory aversive conditioning assay.

      Flies expressing CaMKIIT306A.T307A.Scer\UAS under the control of either Scer\GAL4ey-OK107 or Scer\GAL4c305a have significantly reduced anesthesia-resistant memory (measured 24 hours after 5 cycles of massed training) in an olfactory aversive conditioning assay.

      Flies expressing CaMKIIT306A.T307A.Scer\UAS under the control of Scer\GAL4Mef2.247 show normal anesthesia-resistant memory (measured 24 hours after 5 cycles of massed training) in an olfactory aversive conditioning assay.

      (Malik et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      CaMKIIT306A.T307A.UAS, Scer\GAL4c305a has abnormal memory phenotype, non-suppressible by CASKUAS.cBa, Scer\GAL4c305a

      (Malik et al., 2013)
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      CaMKIIT306A.T307A.UAS, Scer\GAL4c305a is a non-suppressor of abnormal memory phenotype of CASKUAS.cBa, Scer\GAL4c305a

      (Malik et al., 2013)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of CASKScer\UAS.cBa and CaMKIIT306A.T307A.Scer\UAS under the control of Scer\GAL4ey-OK107 is sufficient to rescue the reduction in middle-term memory (measured 3 hours after one cycle of training) seen in an olfactory aversive conditioning assay when either CASKScer\UAS.cBa or CaMKIIT306A.T307A.Scer\UAS is expressed alone under the control of Scer\GAL4ey-OK107.

      Co-expression of CASKScer\UAS.cBa and CaMKIIT306A.T307A.Scer\UAS under the control of Scer\GAL4c305a does not rescue the reduction in middle-term memory (measured 3 hours after one cycle of training) seen in an olfactory aversive conditioning assay when either CASKScer\UAS.cBa or CaMKIIT306A.T307A.Scer\UAS is expressed alone under the control of Scer\GAL4c305a.

      (Malik et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CaMKIIT306A.T307A.Scer\UAS
      CaMKIIT306A.T307A.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)