FlyBase Allele Report: Dmel\Abl[KN.UAS]

General Information

Symbol

Dmel\Abl^KN.UAS

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Hsouna

FlyBase ID

FBal0285361

Feature type

allele

Associated gene

Dmel\Abl

Associated Insertion(s)

Carried in Construct

P{UAS-Abl.KN}

Also Known As

UAS-AblKN

Key Links

Transgenic product class

dominant_negative_variant

(Hsouna et al., 2003)

missense_variant

(Hsouna et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Hsouna et al., 2003)

missense_variant

(Hsouna et al., 2003)

Mutagen

in vitro construct

(Hsouna et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-Abl.KN}

Cytology

Description

UASt regulatory sequences drive expression of a kinase-inactive (K417N) Abl mutant.

(Hsouna et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Abl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^elav.PLu

(Hsouna et al., 2003)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^ftz.ng

(Hsouna et al., 2003)

Phenotype Manifest In

larval longitudinal connective | embryonic stage, with Scer\GAL4^elav.PLu

(Hsouna et al., 2003)

larval longitudinal connective | embryonic stage, with Scer\GAL4^ftz.ng

(Hsouna et al., 2003)

Detailed Description

Statement

Reference

Pan-neural expression of Abl^KN.Scer\UAS under the control of Scer\GAL4^insc-Mz1407 has no embryonic commissural phenotype.

(Dorsten et al., 2010)

Expression of Abl^KN.Scer\UAS using Scer\GAL4^ftz.ng or Scer\GAL4^elav.PLu causes at least one axon bundle to cross the midline inappropriately in 11-20% of embryos examined.

Co-expression of Abl^Scer\UAS.cHa and Abl^KN.Scer\UAS in the Scer\GAL4^ftz.ng pattern suppresses all abnormal axonal crossovers.

Co-expression of Abl^ftz.PH and Abl^KN.Scer\UAS suppresses all abnormal axonal crossovers.

(Hsouna et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^insc-Mz1407/Abl^KN.UAS is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of fra³/fra⁴

(Dorsten et al., 2010)

Abl^KN.UAS/Scer\GAL4^ftz.ng is an enhancer of abnormal neuroanatomy | embryonic stage 16 | dominant phenotype of robo1¹

(Hsouna et al., 2003)

Suppressor of

Statement

Reference

Abl^KN.UAS/Scer\GAL4^elav.PLu is a suppressor of abnormal neuroanatomy phenotype of comm¹

(Hsouna et al., 2003)

Abl^KN.UAS/Scer\GAL4^ftz.ng is a suppressor of abnormal neuroanatomy phenotype of comm¹

(Hsouna et al., 2003)

Other

Statement

Reference

Abl^KN.UAS, Scer\GAL4^insc-Mz1407, fra³/fra⁴ has abnormal neuroanatomy | embryonic stage phenotype

(Dorsten et al., 2010)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^insc-Mz1407/Abl^KN.UAS is an enhancer of larval ventral nerve cord commissure phenotype of fra³/fra⁴

(Dorsten et al., 2010)

Abl^KN.UAS/Scer\GAL4^ftz.ng is an enhancer of larval longitudinal connective | embryonic stage 16 phenotype of robo1¹

(Hsouna et al., 2003)

Suppressor of

Statement

Reference

Abl^KN.UAS/Scer\GAL4^elav.PLu is a suppressor of symmetrical commissure phenotype of comm¹

(Hsouna et al., 2003)

Abl^KN.UAS/Scer\GAL4^ftz.ng is a suppressor of symmetrical commissure phenotype of comm¹

(Hsouna et al., 2003)

Other

Statement

Reference

Abl^KN.UAS, Scer\GAL4^insc-Mz1407, fra³/fra⁴ has tract | ectopic | embryonic stage phenotype

(Dorsten et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Pan-neural expression of Abl^KN.Scer\UAS under the control of Scer\GAL4^insc-Mz1407 in fra³ heterozygotes has no embryonic commissural phenotype.

Pan-neural expression of Abl^KN.Scer\UAS under the control of Scer\GAL4^insc-Mz1407 in fra³/fra⁴ embryos significantly increases the frequency of thin and missing posterior commissure defects.

Less than 10% of fra³/fra⁴ embryos expressing Scer\GAL4^insc-Mz1407>Abl^KN.Scer\UAS exhibit defects characterised by Fas2-positive axons ectopically exiting the central nervous system (CNS).

(Dorsten et al., 2010)

Compared with 26% of heterozygous robo¹ mutants exhibiting inappropriate crossovers, expression of Abl^KN.Scer\UAS in the Scer\GAL4^ftz.ng pattern results in almost all (95%) embryos exhibiting several axon bundles crossing the midline.

Pan-neural expression of Abl^KN.Scer\UAS under the control of Scer\GAL4^elav.PLu partially suppresses the comm¹ commissure phenotype.

Expression of Abl^KN.Scer\UAS in a subset of neurons under the control of Scer\GAL4^ftz.ng partially suppresses the comm¹ commissure phenotype.

(Hsouna et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Abl^KN.Scer\UAS

Abl^KN.UAS

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Hsouna

Secondary FlyBase IDs

References (2)

Report Sections

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