Adult flies that constitutively express Zzzz\aptamerHsf.X8.Scer\UAS display phenotypic abnormalities in the abdominal segments in 8% in cases), wing shape (90% exhibit notched wings) and morphology (20% exhibit crinkled wings), and bristles (33%) and eye protrusions.
Scer\GAL4αTub84B.PL, Zzzz\aptamerHsf.X8.UAS has increased mortality phenotype, suppressible by HsfUAS.GFP, Scer\GAL4αTub84B.PL
Scer\GAL4αTub84B.PL, Zzzz\aptamerHsf.X8.UAS has wing phenotype, non-suppressible by HsfUAS.GFP, Scer\GAL4αTub84B.PL
Zzzz\aptamerHsf.X8.UAS/Scer\GAL4αTub84B.PL is a suppressor of salivary gland phenotype of HsfUAS.GFP, Scer\GAL4c739
Zzzz\aptamerHsf.X8.UAS/Scer\GAL4αTub84B.PL is a suppressor of eye phenotype of Raf98
Zzzz\aptamerHsf.X8.UAS/Scer\GAL4αTub84B.PL is a suppressor of ommatidium phenotype of Raf98
Ubiquitous expression of HsfScer\UAS.T:Avic\GFP under the control of Scer\GAL4αTub84B.PL suppresses the lethality found in Zzzz\aptamerHsf.X8.Scer\UAS expressing animals. The wing notching defect found in these flies is not affected by HsfScer\UAS.T:Avic\GFP expression.
Co-expression of Zzzz\aptamerHsf.X8.Scer\UAS with HsfScer\UAS.T:Avic\GFP in the salivary glands (under the control of Scer\GAL4c739) restores salivary gland morphology to nearly wild-type size.
The high frequencies of lethality seen in flies with high-level systemic HsfScer\UAS.T:Avic\GFP expression (under the control of Scer\GAL4αTub84B.PL) is effectively suppressed by Zzzz\aptamerHsf.X8.Scer\UAS co-expression, resulting in viable and fertile animals.
The addition of Zzzz\aptamerHsf.X8.Scer\UAS to the genetic background of phl98 heterozygotes (under the control of Scer\GAL4αTub84B.PL) results in the strong suppression of the rough eye phenotype that is caused by increased phl98 signaling activity.