FlyBase Allele Report: Dmel\Syx17[LL06330]

General Information

Symbol

Dmel\Syx17^LL06330

Species

D. melanogaster

Name

FlyBase ID

FBal0284931

Feature type

allele

Associated gene

Dmel\Syx17

Associated Insertion(s)

PBac{SAstopDsRed}Syx17^LL06330

Carried in Construct

Also Known As

Syx17[LL], Syx17^LL

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

PBac{SAstopDsRed}Syx17^LL06330

Cytology

Description

Insertion 22 nucleotides downstream of the translation start site.

(Takáts et al., 2013)

Allele components

Component

Use(s)

Inserted element

PBac{SAstopDsRed}

gene trap

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:4,403,852..4,403,852 [-]

(Takáts et al., 2014, Takáts et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage (with Df(3L)Exel8098)

(Takáts et al., 2013)

abnormal locomotor behavior | adult stage (with Syx17^f03584)

(Takáts et al., 2013)

abnormal locomotor behavior | adult stage | nutrition conditional (with Df(3L)Exel8098)

(Takáts et al., 2014)

increased cell death | adult stage (with Df(3L)Exel8098)

(Takáts et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

Syx17^LL06330/Df(3L)Exel8098 eye discs have normal morphology. The cells show accumulation of autophagosomes intracellularly, but secreted autophagosomes are not seen.

(Morelli et al., 2014)

Autolysosome formation is inhibited in the fat body cells of starved Syx17^LL06330/Df(3L)Exel8098 mutant wandering third instar larvae. Climbing defects are seen in adult flies.

The eye colour of Syx17^LL06330/Df(3L)Exel8098 mutant flies is similar to wild type controls.

(Takáts et al., 2014)

Fat body cells of starved Syx17^LL06330/Df(3L)Exel8098 animals accumulate double-membrane autophagosomes and show impaired generation of autolysosomes.

Syx17^LL06330/Syx17^f03584 and Syx17^LL06330/Df(3L)Exel8098 flies show poor climbing ability compared to controls.

Brain neurons of well-fed Syx17^LL06330/Df(3L)Exel8098 flies show large-scale accumulation of autophagosomes and the brains show apoptotic DNA fragmentation.

(Takáts et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Syx17^LL06330/Df(3L)Exel8098 has abnormal locomotor behavior | adult stage phenotype, non-suppressible by Scer\GAL4^elav.PU/Diap1^UAS.cHa

(Takáts et al., 2013)

Syx17^LL06330/Df(3L)Exel8098 has abnormal locomotor behavior | adult stage phenotype, non-suppressible by Scer\GAL4^elav.PU/BacA\p35^UAS.cHa

(Takáts et al., 2013)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of th^Scer\UAS.cHa under the control of Scer\GAL4^elav.PU does not rescue the climbing defects of Syx17^LL06330/Df(3L)Exel8098 flies.

(Takáts et al., 2013)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^elav.PU does not rescue the climbing defects of Syx17^LL06330/Df(3L)Exel8098 flies.

(Takáts et al., 2013)

Complementation and Rescue Data

Rescued by

Syx17^LL06330/Df(3L)Exel8098 is rescued by Syx17^+tgen

(Takáts et al., 2014)

Syx17^LL06330/Df(3L)Exel8098 is rescued by Syx17^{1-301.Tag:FLAG}

(Takáts et al., 2014)

Syx17^LL06330/Df(3L)Exel8098 is rescued by Syx17^{1-270.Tag:FLAG}

(Takáts et al., 2014)

Syx17^LL06330/Df(3L)Exel8098 is rescued by Syx17^+tgen

(Takáts et al., 2013)

Not rescued by

Syx17^LL06330/Df(3L)Exel8098 is not rescued by Syx17^{1-251.mCherry}

(Takáts et al., 2014)

Comments

Expression of Syx17^+tgen rescues the autolysosome formation defects seen in starved Syx17^LL06330/Df(3L)Exel8098 mutant wandering third instar larvae. The climbing defects seen in the resulting adult flies are also rescued.

Expression of Syx17^{1-301.T:Zzzz\FLAG} rescues the autolysosome formation defects seen in starved Syx17^LL06330/Df(3L)Exel8098 mutant wandering third instar larvae. The climbing defects seen in the resulting adult flies are also rescued.

Expression of Syx17^{1-270.T:Zzzz\FLAG} rescues the autolysosome formation defects seen in starved Syx17^LL06330/Df(3L)Exel8098 mutant wandering third instar larvae. The climbing defects seen in the resulting adult flies are also rescued.

Expression of Syx17^{1-251.T:Disc\RFP-mCherry} fails to rescue the autolysosome formation defects seen in starved Syx17^LL06330/Df(3L)Exel8098 mutant wandering third instar larvae. The climbing defects seen in the resulting adult flies are also not rescued.

(Takáts et al., 2014)

Syx17^+tgen rescues the climbing defects of Syx17^LL06330/Df(3L)Exel8098 flies.

(Takáts et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

140948

y^* w^*; PBac{SAstopDsRed}Syx17^LL06330 P{FRT(w^hs)}2A P{neoFRT}82B P{Car20y}96E/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Stx17^LL06330

(Murakawa et al., 2020)

Stx17^LL

(Murakawa et al., 2020)

Syx17^LL06330

(Manzéger et al., 2021, Takáts et al., 2021, Ikeda et al., 2019, Csizmadia et al., 2018, Hegedűs et al., 2016, Takáts et al., 2015, Morelli et al., 2014, Takáts et al., 2014)

Syx17^LL

(Krzystek et al., 2023, Rong et al., 2022, Hegedűs et al., 2016)

Syx17LL06330

(Morelli et al., 2016)

Syx17[LL06330]

(Boda et al., 2019)

Syx17[LL]

(Takáts et al., 2021, Boda et al., 2019, Takáts et al., 2014, Takáts et al., 2013)

syx17^LL

(Neisch et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

Open Close