FB2024_03 , released June 25, 2024
Allele: Dmel\lbeeve.SS
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General Information
Symbol
Dmel\lbeeve.SS
Species
D. melanogaster
Name
FlyBase ID
FBal0284521
Feature type
allele
Associated gene
Dmel\lbe
Associated Insertion(s)
Carried in Construct
P{eve-lbe.SS}
Key Links
Transgenic product class
cDNA
(Buechling et al., 2009)
wild_type
(Buechling et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Buechling et al., 2009)
wild_type
(Buechling et al., 2009)
Mutagen
in vitro construct
(Buechling et al., 2009)
Progenitor genotype
Carried in construct
P{eve-lbe.SS}
Cytology
Description

The genomic eve mesodermal enhancer (eme) fragment (SphI +5.8 kb and StuI +6.6 kb, eme900), the HSP70 promoter and the lbe cDNA flanked by the 3' and 5' UTRs from eve.

(Buechling et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
eve
Encoded product / tool
lbe
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      lbeeve.SS larvae have a reduced number of pericardial cells.

      Morphogenesis of the myocardium is moderately affected in lbeeve.SS embryos. Disorganization and loss of dorsal skeletal muscles is also observed.

      There is a significant reduction in heart diameter in 1 week old lbeeve.SS adult flies.

      Diastolic and systolic diameters of live lbeeve.SS adult hearts in A4 abdominal segments are significantly reduced compared to controls. This effect is less pronounced in A3 segments, while it is little changed in the conical chamber in A2.

      Adult lbeeve.SS hearts show a normal, regular arrangement of transverse myofibrils within the tin-expressing myocardium. In contrast, the spacing of Z-lines and the parallel arrangement of myofibrils are significantly defective in the longitudinal heart-associated muscles of lbeeve.SS hearts.

      The following cardiac phenotypes are observed in lbeeve.SS flies: 1) non-contractile asystolic heart periods or temporary cardiac arrest; 2) double or multiple beats within otherwise regular periods; 3) varying duration of diastolic intervals resulting in arrhythmias.

      (Buechling et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Scer\GAL4eve.eme-mediated expression of eveScer\UAS.cBa rescues the reduced number of pericardial cells in lbeeve.SS larvae.

      Scer\GAL4eve.eme-mediated expression of eveScer\UAS.cBa rescues the reduced heart diameter of lbeeve.SS adults, and partially rescues the reduced diastolic and systolic diameters seen in live lbeeve.SS hearts.

      Scer\GAL4eve.eme-mediated expression of eveScer\UAS.cBa rescues the myofibrillar structure defects of lbeeve.SS adult hearts.

      Scer\GAL4eve.eme-mediated expression of eveScer\UAS.cBa rescues the asystolic events and arrhythmias seen in lbeeve.SS adult hearts.

      (Buechling et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      lbeeve.SS is rescued by lbeGD4157/Scer\GAL4eve.eme

      (Buechling et al., 2009)
      Comments

      Scer\GAL4eve.eme-mediated expression of lbeGD4157 rescues the reduced heart diameter of lbeeve.SS adults, and partially rescues the reduced diastolic and systolic diameters seen in live lbeeve.SS hearts.

      Scer\GAL4eve.eme-mediated expression of lbeGD4157 rescues the myofibrillar structure defects of lbeeve.SS adult hearts.

      (Buechling et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      lbeeve.SS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)