FlyBase Allele Report: Dmel\PIP4K[29]

General Information

Symbol

Dmel\PIP4K²⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0284009

Feature type

allele

Associated gene

Dmel\PIP4K

Associated Insertion(s)

TI{TI}PIP4K²⁹

Carried in Construct

Also Known As

dPIP4K²⁹

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Gupta et al., 2013)

Mutagen

ends-out gene targeting

(Gupta et al., 2013)

Progenitor genotype

Associated Insertion(s)

TI{TI}PIP4K²⁹

Cytology

Description

The PIP4K kinase domain has been disrupted by insertion of sequence comprising stop codons in all three reading frames and a w^+mW.hs marker.

(Gupta et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage

(Gupta et al., 2013)

abnormal developmental rate | larval stage | nutrition conditional

(Sharma et al., 2019)

abnormal size | pupal stage | conditional

(Kamalesh et al., 2017)

decreased body size | larval stage

(Gupta et al., 2013)

decreased cell size | larval stage

(Gupta et al., 2013)

Phenotype Manifest In

embryonic/larval hemocyte | third instar larval stage

(Kamalesh et al., 2017)

embryonic/larval salivary gland | larval stage

(Gupta et al., 2013)

multivesicular body | pupal stage | conditional

(Kamalesh et al., 2017)

photoreceptor neuron | pupal stage | conditional

(Kamalesh et al., 2017)

vesicle | pupal stage | conditional

(Kamalesh et al., 2017)

Detailed Description

Statement

Reference

PIP4K²⁹ individuals show delayed pupariation under normal diet and earlier pupariation under high sugar diet, as compared to controls.

(Sharma et al., 2019)

The photoreceptors of PIP4K²⁹ homozygous late pupae (i.e. at 90% of pupal development) reared in light conditions exhibit significant increases in the number and the size of endosome-like vesicles, a significant expansion of multi-vesicular bodies in the cell bodies, and upregulation of early endosomes (assessed by EEA1 or Rab5 Western blot and/or immuno-staining), but do not exhibit obvious defects in the distribution of endoplasmic reticulum (identified by KDEL immuno-staining) and of the Golgi apparatus (identified by GM130 immuno-staining), as compared to controls; these mutants reared in light conditions do not exhibit any obvious defects in the structure of rhabdomeres, or in the levels of autophagosomes in head extracts (assessed by Atg8a protein levels by Western blot or by puncta of fluorescent human LC3 protein), as compared to controls. The haemocytes of PIP4K²⁹ homozygous third instar larvae display a significant increase in clathrin-dependent endocytosis, as compared to controls.

(Kamalesh et al., 2017)

Light responses are normal in PIP4K²⁹ photoreceptors.

(Chakrabarti et al., 2015)

Homozygous adults have phosphatidylinositol 5-phosphate levels double that of wild-type controls, while the levels of phosphatidylinositol 4-phosphate and phosphatidylinositol (4,5)-bisphosphate are not reduced compared to wild type.

Homozygous larvae show developmental defects. Although they have the same body weight as controls immediately after hatching, they weigh less than wild-type controls at multiple points during development, with the maximum difference being at 60 hours after hatching at 22[o]C. The mutant larvae show a delay in pupariation compared to wild type. Salivary gland cells are reduced in size in the mutant larvae compared to wild type.

(Gupta et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

PIP4K²⁹ is an enhancer of increased cell size | third instar larval stage phenotype of Scer\GAL4^AB1, chico^UAS.cUa

(Sharma et al., 2019)

PIP4K²⁹ is an enhancer of increased cell size | third instar larval stage phenotype of InR^UAS.Exel, Scer\GAL4^AB1

(Sharma et al., 2019)

NOT Enhancer of

Statement

Reference

PIP4K²⁹ is a non-enhancer of increased cell size | third instar larval stage phenotype of Pdk1^A467V.UAS, Scer\GAL4^AB1

(Sharma et al., 2019)

PIP4K²⁹ is a non-enhancer of decreased cell size | third instar larval stage phenotype of Rheb^HMS00923, Scer\GAL4^AB1

(Sharma et al., 2019)

PIP4K²⁹ is a non-enhancer of abnormal neurophysiology phenotype of rdgB⁹

(Chakrabarti et al., 2015)

NOT Suppressor of

Statement

Reference

PIP4K²⁹ is a non-suppressor of increased cell size | third instar larval stage phenotype of Pdk1^A467V.UAS, Scer\GAL4^AB1

(Sharma et al., 2019)

PIP4K²⁹ is a non-suppressor of increased cell size | third instar larval stage phenotype of Scer\GAL4^AB1, Tsc1^HMC03672

(Sharma et al., 2019)

PIP4K²⁹ is a non-suppressor of decreased cell size | third instar larval stage phenotype of Rheb^HMS00923, Scer\GAL4^AB1

(Sharma et al., 2019)

Other

Statement

Reference

PIP4K²⁹, Rheb^UAS.cPa, Scer\GAL4^AB1 has increased cell size | third instar larval stage phenotype

(Sharma et al., 2019)

Phenotype Manifest In

Suppressed by

Statement

Reference

PIP4K²⁹ has multivesicular body | pupal stage | conditional phenotype, suppressible by Scer\GAL4^ninaE.PU/Rab5^{S43N.UASp.YFP}

(Kamalesh et al., 2017)

PIP4K²⁹ has multivesicular body | pupal stage | conditional phenotype, suppressible by Scer\GAL4^ninaE.PU/Rheb^UAS.cPa

(Kamalesh et al., 2017)

PIP4K²⁹ has photoreceptor neuron | pupal stage | conditional phenotype, suppressible by Scer\GAL4^ninaE.PU/Rab5^{S43N.UASp.YFP}

(Kamalesh et al., 2017)

PIP4K²⁹ has vesicle | pupal stage | conditional phenotype, suppressible by Scer\GAL4^ninaE.PU/Rab5^{S43N.UASp.YFP}

(Kamalesh et al., 2017)

PIP4K²⁹ has vesicle | pupal stage | conditional phenotype, suppressible by Scer\GAL4^ninaE.PU/Rheb^UAS.cPa

(Kamalesh et al., 2017)

NOT suppressed by

Statement

Reference

PIP4K²⁹ has photoreceptor neuron | pupal stage | conditional phenotype, non-suppressible by Scer\GAL4^ninaE.PU/Rheb^UAS.cPa

(Kamalesh et al., 2017)

Enhancer of

Statement

Reference

PIP4K²⁹ is an enhancer of fat cell | third instar larval stage phenotype of InR^UAS.Exel, Scer\GAL4^AB1

(Sharma et al., 2019)

PIP4K²⁹ is an enhancer of fat cell | third instar larval stage phenotype of Scer\GAL4^AB1, chico^UAS.cUa

(Sharma et al., 2019)

NOT Enhancer of

Statement

Reference

PIP4K²⁹ is a non-enhancer of fat cell | third instar larval stage phenotype of Pdk1^A467V.UAS, Scer\GAL4^AB1

(Sharma et al., 2019)

PIP4K²⁹ is a non-enhancer of fat cell | third instar larval stage phenotype of Rheb^HMS00923, Scer\GAL4^AB1

(Sharma et al., 2019)

PIP4K²⁹ is a non-enhancer of eye photoreceptor cell phenotype of rdgB⁹

(Chakrabarti et al., 2015)

PIP4K²⁹ is a non-enhancer of retina | conditional phenotype of rdgB⁹

(Chakrabarti et al., 2015)

NOT Suppressor of

Statement

Reference

PIP4K²⁹ is a non-suppressor of fat cell | third instar larval stage phenotype of Pdk1^A467V.UAS, Scer\GAL4^AB1

(Sharma et al., 2019)

PIP4K²⁹ is a non-suppressor of fat cell | third instar larval stage phenotype of Scer\GAL4^AB1, Tsc1^HMC03672

(Sharma et al., 2019)

PIP4K²⁹ is a non-suppressor of fat cell | third instar larval stage phenotype of Rheb^HMS00923, Scer\GAL4^AB1

(Sharma et al., 2019)

Other

Statement

Reference

PIP4K²⁹, Rheb^UAS.cPa, Scer\GAL4^AB1 has fat cell | third instar larval stage phenotype

(Sharma et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

PIP4K²⁹ third instar larvae expressing Rheb^UAS.cPa under the control of Scer\GAL4^AB1 show significantly bigger salivary gland cells, as compared to PIP4K²⁹ single mutants.

(Sharma et al., 2019)

The expansion in endosome-like vesicles and multivesicular bodies observed in photoreceptors of PIP4K²⁹ homozygous late pupae reared in light conditions are suppressed by the expression of Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP}, but not Rheb^Scer\UAS.cPa, under the control of Scer\GAL4^ninaE.PU.

(Kamalesh et al., 2017)

PIP4K²⁹ does not exacerbate the reduced electrical response to light and retinal degeneration phenotypes of rdgB⁹.

(Chakrabarti et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

PIP4K²⁹ is rescued by PIP4K^UAS.cSa/Scer\GAL4^Act5C.PI

(Sharma et al., 2019)

PIP4K²⁹ is rescued by Scer\GAL4^ninaE.PU/PIP4K^UAS.EGFP

(Kamalesh et al., 2017)

PIP4K²⁹ is rescued by Scer\GAL4^da.G32/PIP4K^UAS.EGFP

(Gupta et al., 2013)

Not rescued by

PIP4K²⁹ is not rescued by PIP4K^KD.UAS/Scer\GAL4^Act5C.PI

(Sharma et al., 2019)

Comments

The increases in endosome-like vesicles and in multi-vesicular bodies observed in photoreceptors of PIP4K²⁹ homozygous late pupae reared in light conditions are rescued by the expression of PIP4K^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^ninaE.PU.

(Kamalesh et al., 2017)

The reduced body weight and reduced salivary gland cell size of PIP4K²⁹ larvae relative to wild type is rescued by expression of PIP4K^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32.

(Gupta et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

PIP4K²⁹

(Kamalesh et al., 2017)

dPIP4K²⁹

(Ghosh et al., 2023, Mathre et al., 2019, Sharma et al., 2019, Chakrabarti et al., 2015, Gupta et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (6)

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