Amino acid replacement: E279K.
Nucleotide substitution: G835A.
Adults carrying fz8A4.Act5C in a fz21 background have an average of 3.3 +/- 0.8 extra tarsal joints in the leg compared to wild type.
fz8A4.Act5C rescues the wing margin phenotype of fz21 fz2C1 double homozygous clones.