FlyBase Allele Report: Hsap\GFAP[UAS.cWa]

General Information

Symbol

Hsap\GFAP^UAS.cWa

Species

H. sapiens

Name

FlyBase ID

FBal0283569

Feature type

allele

Associated gene

Hsap\GFAP

Associated Insertion(s)

Carried in Construct

P{UAS-hGFAP.W}

Key Links

Transgenic product class

wild_type

(Wang et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Wang et al., 2011)

Mutagen

in vitro construct

(Wang et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-hGFAP.W}

Cytology

Description

UAS regulatory sequences drive expression of wild-type Hsap\GFAP coding sequences.

(Wang et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\GFAP

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^repo.PU

(Wang et al., 2015)

increased cell death | progressive, with Scer\GAL4^repo

(Wang et al., 2011)

paralytic, with Scer\GAL4^repo

(Wang et al., 2011)

Phenotype Manifest In

adult brain, with Scer\GAL4^repo

(Wang et al., 2011)

adult CNS glial cell, with Scer\GAL4^repo.PU

(Wang et al., 2015)

lamina, with Scer\GAL4^repo

(Wang et al., 2011)

neuron | cell non-autonomous, with Scer\GAL4^repo

(Wang et al., 2011)

Detailed Description

Statement

Reference

Over-expression of two copies (but not one) of Hsap\GFAP^Scer\UAS.cWa driven in glia by Scer\GAL4^repo.PU leads to Rosenthal fiber-like inclusion formations in 20 day old flies.

(Wang et al., 2015)

Expression of Hsap\GFAP^Scer\UAS.cWa under the control of Scer\GAL4^repo does not affect developmental rate or eclosion. Overall brain structure appears normal at 1 day of age, as assayed by haematoxylin and eosin. A TUNEL assay for apoptotic cells does not reveal dying cells at 1 day after eclosion. However, increasing numbers of TUNEL-positive cells are seen as the animals age, indicating some limited toxicity. The TUNEL-positive cells are widespread, with no obvious predilection for particular areas of the brain. In the lamina, there is a trend towards decreased numbers of glial cells with advancing age and a significant loss of neurons at 10 and 20 days of age, consistent with a non-autonomous effect.

Expression of Hsap\GFAP^Scer\UAS.cWa in glial under the control of Scer\GAL4^repo promotes the loss of GluRIIB-expressing neurons which is particularly apparent by 20 days after eclosion.

Flies expressing Hsap\GFAP^Scer\UAS.cWa in glial under the control of Scer\GAL4^repo exhibit a significantly increased frequency of seizures in response to mechanical stimulation.

Flies expressing Hsap\GFAP^Scer\UAS.cWa in glial under the control of Scer\GAL4^repo display some abnormal protein aggregation (although less than in Hsap\GFAP^{R79H.Scer\UAS} mutants), with numerous eosinophilic, elongated and beaded inclusions. The number of inclusions increases with age.

Flies expressing Hsap\GFAP^Scer\UAS.cWa in glial under the control of Scer\GAL4^repo display an increase in autophagy induction with age, with a modest induction present at 1 day after eclosion and more substantial induction at 10 and 20 days of age.

(Wang et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has increased cell death | progressive phenotype, enhanceable by Catⁿ¹/Cat[+]

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has paralytic phenotype, enhanceable by Catⁿ¹/Cat[+]

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has increased cell death | progressive phenotype, enhanceable by Sod1ⁿ¹/Sod[+]

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has paralytic phenotype, enhanceable by Sod1ⁿ¹/Sod[+]

(Wang et al., 2011)

Suppressed by

Statement

Reference

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has increased cell death | progressive phenotype, suppressible by l(2)efl^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has paralytic phenotype, suppressible by l(2)efl^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has increased cell death | progressive phenotype, suppressible by Hsp26^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has paralytic phenotype, suppressible by Hsp26^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has increased cell death | progressive phenotype, suppressible by Hsp27^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has paralytic phenotype, suppressible by Hsp27^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has increased cell death | progressive phenotype, suppressible by Cat^UAS.cAa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has paralytic phenotype, suppressible by Cat^UAS.cAa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has increased cell death | progressive phenotype, suppressible by Hsap\HSPA1A^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has paralytic phenotype, suppressible by Hsap\HSPA1A^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has increased cell death | progressive phenotype, suppressible by Hsap\SOD1^UAS.cWb, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has paralytic phenotype, suppressible by Hsap\SOD1^UAS.cWb, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has increased cell death | progressive phenotype, suppressible by Eaat1^UAS.cRa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has paralytic phenotype, suppressible by Eaat1^UAS.cRa, Scer\GAL4^repo

(Wang et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has lamina phenotype, enhanceable by Catⁿ¹/Cat[+]

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has adult brain phenotype, enhanceable by Catⁿ¹/Cat[+]

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has neuron | cell non-autonomous phenotype, enhanceable by Catⁿ¹/Cat[+]

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has lamina phenotype, enhanceable by Sod1ⁿ¹/Sod[+]

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has adult brain phenotype, enhanceable by Sod1ⁿ¹/Sod[+]

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has neuron | cell non-autonomous phenotype, enhanceable by Sod1ⁿ¹/Sod[+]

(Wang et al., 2011)

Suppressed by

Statement

Reference

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has lamina phenotype, suppressible by l(2)efl^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has adult brain phenotype, suppressible by l(2)efl^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has adult brain phenotype, suppressible by Cat^UAS.cAa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has neuron | cell non-autonomous phenotype, suppressible by Cat^UAS.cAa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has lamina phenotype, suppressible by Hsap\HSPA1A^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has adult brain phenotype, suppressible by Hsap\HSPA1A^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has neuron | cell non-autonomous phenotype, suppressible by Hsap\HSPA1A^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has lamina phenotype, suppressible by Hsap\SOD1^UAS.cWb, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has adult brain phenotype, suppressible by Hsap\SOD1^UAS.cWb, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has neuron | cell non-autonomous phenotype, suppressible by Hsap\SOD1^UAS.cWb, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has lamina phenotype, suppressible by Eaat1^UAS.cRa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has adult brain phenotype, suppressible by Eaat1^UAS.cRa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has neuron | cell non-autonomous phenotype, suppressible by l(2)efl^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has neuron | cell non-autonomous phenotype, suppressible by Eaat1^UAS.cRa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has lamina phenotype, suppressible by Hsp26^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has adult brain phenotype, suppressible by Hsp26^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has neuron | cell non-autonomous phenotype, suppressible by Hsp26^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has lamina phenotype, suppressible by Hsp27^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has adult brain phenotype, suppressible by Hsp27^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has neuron | cell non-autonomous phenotype, suppressible by Hsp27^UAS.cWa, Scer\GAL4^repo

(Wang et al., 2011)

Hsap\GFAP^UAS.cWa, Scer\GAL4^repo has lamina phenotype, suppressible by Cat^UAS.cAa, Scer\GAL4^repo

(Wang et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Overexpression of l(2)efl^Scer\UAS.cWa in flies expressing Hsap\GFAP^Scer\UAS.cWa under the control of Scer\GAL4^repo results in a significant reduction in cell death. In addition, expression of l(2)efl^Scer\UAS.cWa also decreases the number of seizures. Protection from cellular toxicity is accompanied by a significant reduction in the number of inclusion bodies and in activation of autophagy.

Overexpression of Hsp26^Scer\UAS.cWa in flies expressing Hsap\GFAP^Scer\UAS.cWa under the control of Scer\GAL4^repo results in a significant reduction in cell death. In addition, expression of Hsp26^Scer\UAS.cWa also decreases the number of seizures. Protection from cellular toxicity is accompanied by a significant reduction in the number of inclusion bodies.

Overexpression of Hsp27^Scer\UAS.cWa in flies expressing Hsap\GFAP^Scer\UAS.cWa under the control of Scer\GAL4^repo results in a significant reduction in cell death. In addition, expression of Hsp27^Scer\UAS.cWa also decreases the number of seizures. Protection from cellular toxicity is accompanied by a significant reduction in the number of inclusion bodies.

Overexpression of Cat^Scer\UAS.cAa in flies expressing Hsap\GFAP^Scer\UAS.cWa under the control of Scer\GAL4^repo results in a significant reduction in cell death. In addition, expression of Cat^Scer\UAS.cAa also decreases the number of seizures. Protection from cellular toxicity does not alter the number of inclusion bodies.

A Catⁿ¹ heterozygous background significantly enhances Hsap\GFAP^Scer\UAS.cWa toxicity and increases the frequency of mechanically-stimulated seizures in these flies. An increase in cellular toxicity does not alter the number of inclusion bodies but does increase autophagy induction.

A Sodⁿ¹ heterozygous background significantly enhances Hsap\GFAP^Scer\UAS.cWa toxicity and increases the frequency of mechanically-stimulated seizures in these flies. Protection from cellular toxicity does not alter the number of inclusion bodies. An increase in cellular toxicity does not alter the number of inclusion bodies but does increase autophagy induction.

Overexpression of Hsap\HSPA1A^Scer\UAS.cWa in flies expressing Hsap\GFAP^Scer\UAS.cWa under the control of Scer\GAL4^repo results in a significant reduction in cell death. In addition, expression of Hsap\HSPA1A^Scer\UAS.cWa also decreases the number of seizures. Protection from cellular toxicity is accompanied by a significant reduction in the number of inclusion bodies and in activation of autophagy.

Overexpression of Hsap\SOD1^Scer\UAS.cWb in flies expressing Hsap\GFAP^Scer\UAS.cWa under the control of Scer\GAL4^repo results in a significant reduction in cell death. In addition, expression of Hsap\SOD1^Scer\UAS.cWb also decreases the number of seizures. Protection from cellular toxicity does not alter the number of inclusion bodies.

Expression of Eaat1^Scer\UAS.cRa significantly suppresses the Hsap\GFAP toxicity, but does not influence inclusion formation, Hsap\GFAP solubility or autophagy induction.

(Wang et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\GFAP^Scer\UAS.cWa

Hsap\GFAP^UAS.cWa

Name Synonyms

Secondary FlyBase IDs

References (2)

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