FB2024_03 , released June 25, 2024
Allele: Dmel\FASN3RNAi.WIZ.UAS
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General Information
Symbol
Dmel\FASN3RNAi.WIZ.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0283269
Feature type
allele
Associated gene
Dmel\FASN3
Associated Insertion(s)
Carried in Construct
P{UAS-FASN3.RNAi.WIZ}
Key Links
Transgenic product class
RNAi_reagent
(Parvy et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Parvy et al., 2012)
Mutagen
in vitro construct
(Parvy et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-FASN3.RNAi.WIZ}
Cytology
Description

UAS regulatory sequences drive expression of an inverted repeat of FASN3 sequence.

(Parvy et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
FASN3
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Whereas Scer\GAL4da.PU-mediated expression of FASN3dsRNA.WIZ.Scer\UAS (plus Dcr-2Scer\UAS.cDa) is lethal at the larval L2 stage, co-expression of Scer\GAL80svp.3kb which inhibits Scer\GAL4 in the oenocytes results in viable flies.

      (Garrido et al., 2015)

      Flies expressing FASN3dsRNA.WIZ.Scer\UAS under the control of Scer\GAL4insc-Mz1407 are extremely sensitive to dessication, with significantly decreased survival time during dessication stress, as compared to controls. In mating choice assays, wild type flies do not exhibit a significant difference in preference for opposite sex flies expressing FASN3dsRNA.WIZ.Scer\UAS under the control of Scer\GAL4insc-Mz1407 versus controls.

      (Wicker-Thomas et al., 2015)

      Expression of CG17374dsRNA.WIZ.Scer\UAS under the control of Scer\GAL4sal.BO results in lethality at the L2/L3 larval transition.

      Larvae expressing CG17374dsRNA.WIZ.Scer\UAS under the control of Scer\GAL4sal.BO and transferred to blue tinted food medium at the L2 or early L3 stage show blue staining of the spiracles that sometimes extends into the main tracheal trunks, indicating a water-tightness defect in the spiracles.

      (Parvy et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      CG17374dsRNA.WIZ.Scer\UAS
      FASN3RNAi.WIZ.UAS
      FASN3dsRNA.WIZ.Scer\UAS
      FASN3dsRNA.WIZ.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (5)