UAS drives expression of E(spl)m8-HLH in which a STOP codon is substituted in place of the W176 of the WRPW motif, thus removing this motif and abolishing gro protein binding. In addition, S159 is changed to A to prevent phosphorylation.
Expression of E(spl)m8-HLHSA.Δgro.Scer\UAS with Scer\GAL4sca-537.4 has no effect on the eye or interommatidial bristles or scutellar macrochaetes.
Expression of E(spl)m8-HLHSA.Δgro.Scer\UAS with Scer\GAL4455.2 has no effect on scutellar macrochaetes.
E(spl)m8-HLHSA.Δgro.UAS/Scer\GAL4h-H10 is a non-enhancer of eye phenotype of Nspl-1
E(spl)m8-HLHSA.Δgro.UAS/Scer\GAL4sca-109-68 is a non-enhancer of eye phenotype of Nspl-1
E(spl)m8-HLHSA.Δgro.UAS/Scer\GAL4h-H10 is a non-suppressor of eye phenotype of Nspl-1
E(spl)m8-HLHSA.Δgro.UAS/Scer\GAL4sca-109-68 is a non-suppressor of eye phenotype of Nspl-1
E(spl)m8-HLHSA.Δgro.UAS/Scer\GAL4h-H10 is a non-suppressor of scutellar bristle | ectopic phenotype of Nspl-1
E(spl)m8-HLHSA.Δgro.UAS/Scer\GAL4h-H10 is a non-suppressor of scutellar bristle phenotype of Nspl-1