FB2024_03 , released June 25, 2024
Allele: Scer\GAL4ple.TH-D
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General Information
Symbol
Scer\GAL4ple.TH-D
Species
S. cerevisiae
Name
FlyBase ID
FBal0282521
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{ple-GAL4.TH-D}
Also Known As
TH-D-Gal4, TH-D1-Gal4, THD1GAL4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Liu et al., 2012)
Progenitor genotype
Carried in construct
P{ple-GAL4.TH-D}
Cytology
Description

A sub-fragment of the genomic ple sequence present in Scer\GAL4ple.PF is fused upstream of the GAL4 coding region.

(Liu et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
ple_TH-D
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      dopaminergic PPM2 neuron | subset
      (Xie et al., 2018, Pathak et al., 2015, Liu et al., 2012)
      dopaminergic PPM3 neuron
      (Liu et al., 2012)
      dopaminergic PPL1 neuron | subset
      (Xie et al., 2018, Pathak et al., 2015, Liu et al., 2012)
      dopaminergic PAM neuron | subset
      (Pathak et al., 2015)
      dopaminergic PPM3 neuron | subset
      (Xie et al., 2018, Pathak et al., 2015)
      adult dopaminergic VUM neuron of the gnathal ganglion | subset
      (Pathak et al., 2015)
      dopaminergic neuron | subset
      (Pathak et al., 2015)
      dopaminergic PPL2c neuron | subset
      (Xie et al., 2018)
      Additional Information
      Statement
      Reference

      ScerGAL4ple.TH-D drives expression in a subset of ple-positive dopaminergic neurons in the adult brain as well as in the thorax and abdomen.

      (Pathak et al., 2015)

      ScerGAL4ple.TH-D' drives expression in a subset of the dopaminergic neurons in the adult brain labelled by ScerGAL4ple.PF : around 3 PPM2 neurons, 6 PPM3 neurons and 10 PPL1 neurons. There was also some non-systematic expression in less than 1 neuron in the PAM and PPM1 clusters.

      (Liu et al., 2012)
       
      Marker for
      Reflects expression of
      ple
      (Xie et al., 2018, Liu et al., 2012)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Activation of neurons expressing any of the specific dopaminergic neuron drivers Scer\GAL4ple.TH-D, Scer\GAL4ple.TH-D-4 or Scer\GAL4ple.TH-F-1 through overexpression of temperature sensitive TrpA1 results in increased sleep loss in both mature (7-8 day) flies compared to controls. Expression under the control of Scer\GAL4ple.TH-D or Scer\GAL4ple.TH-D-4 also results in sleep loss in young (1 day old) flies. The resulting Scer\GAL4ple.TH-D-4>TrpA1 mature flies show a deficit in courtship measures and a reduction in VA1v volume. DA1 and VA1d volumes are unaffected. No difference in sleep amount in young flies is seen when neurons expressing Scer\GAL4ple.TH-F-1 are activated. Activating neurons that express in ventral projections to the antennal lobe (AL) in young flies using Scer\GAL4ple.TH-C results in sleep loss, but the flies do not show courtship deficits or glomerular volumetric changes in mature adulthood.

      (Kayser et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Overexpression of pleScer\UAS.cFa encoding the neuronal isoform either through development, or in pupae alone, can rescue flight initiation of Scer\GAL4ple.TH-D>olf186-FE180A.Scer\UAS animals.

      (Pathak et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4ple.TH-D
      Name Synonyms
      Secondary FlyBase IDs
        References (11)