FlyBase Allele Report: Dmel\esg[HMS02538]

General Information

Symbol

Dmel\esg^HMS02538

Species

D. melanogaster

Name

FlyBase ID

FBal0281540

Feature type

allele

Associated gene

Dmel\esg

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS02538}

Also Known As

UAS-esg-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS02538}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS02538

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage, with Scer\GAL4^{Delta-05151-G}

(Li et al., 2017)

decreased cell number | adult stage, with Scer\GAL4^Su(H).GBE

(Li et al., 2017)

increased cell number | adult stage, with Scer\GAL4^{Delta-05151-G}

(Li et al., 2017)

increased cell number | adult stage, with Scer\GAL4^esg.PU

(Li et al., 2017)

Phenotype Manifest In

adult midgut | adult stage, with Scer\GAL4^{Delta-05151-G}

(Li et al., 2017)

adult midgut | adult stage, with Scer\GAL4^esg.PU

(Li et al., 2017)

adult midgut | adult stage, with Scer\GAL4^Su(H).GBE

(Li et al., 2017)

enteroblast | adult stage, with Scer\GAL4^Su(H).GBE

(Li et al., 2017)

enteroendocrine cell | adult stage, with Scer\GAL4^{Delta-05151-G}

(Li et al., 2017)

enteroendocrine cell | adult stage, with Scer\GAL4^esg.PU

(Li et al., 2017)

intestinal stem cell | adult stage, with Scer\GAL4^{Delta-05151-G}

(Li et al., 2017)

Detailed Description

Statement

Reference

Expression of esg^HMS02538 under the control of Scer\GAL4^Dl-05151-G leads to abnormal clusters of cells containing enteroendocrine cells, a significant increase in the ratio of enteroendocrine cells to enterocytes, a loss of intestinal stem cells, but no significant change in the number of dividing cells per gut, and an overall increase in enteroendocrine cells in the midgut, as compared to controls.

Expression of esg^HMS02538 under the control of Scer\GAL4^Su(H).GBE leads to a reduction in the number of enteroblasts in the adult midgut, but no significant difference in the number of enteroendocrine cells, as compared to controls.

Expression of esg^HMS02538 under the control of Scer\GAL4^esg.PU leads to a significant increase in the number of enteroendocrine cells in the adult midgut, as compared to controls.

(Li et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^{Delta-05151-G}, esg^HMS02538 has increased cell number | adult stage phenotype, suppressible by sc^JF02104/Scer\GAL4^{Delta-05151-G}

(Li et al., 2017)

Scer\GAL4^{Delta-05151-G}, esg^HMS02538 has increased cell number | adult stage phenotype, suppressible by ase^RNAi.UAS.cUa/Scer\GAL4^{Delta-05151-G}

(Li et al., 2017)

Scer\GAL4^esg.PU, esg^HMS02538 has increased cell number | adult stage phenotype, suppressible by pros^RNAi.UAS.cUa/Scer\GAL4^esg.PU

(Li et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^{Delta-05151-G}, esg^HMS02538 has adult midgut | adult stage phenotype, suppressible by sc^JF02104/Scer\GAL4^{Delta-05151-G}

(Li et al., 2017)

Scer\GAL4^{Delta-05151-G}, esg^HMS02538 has enteroendocrine cell | adult stage phenotype, suppressible by sc^JF02104/Scer\GAL4^{Delta-05151-G}

(Li et al., 2017)

Scer\GAL4^{Delta-05151-G}, esg^HMS02538 has adult midgut | adult stage phenotype, suppressible by ase^RNAi.UAS.cUa/Scer\GAL4^{Delta-05151-G}

(Li et al., 2017)

Scer\GAL4^{Delta-05151-G}, esg^HMS02538 has enteroendocrine cell | adult stage phenotype, suppressible by ase^RNAi.UAS.cUa/Scer\GAL4^{Delta-05151-G}

(Li et al., 2017)

Scer\GAL4^esg.PU, esg^HMS02538 has adult midgut | adult stage phenotype, suppressible by pros^RNAi.UAS.cUa/Scer\GAL4^esg.PU

(Li et al., 2017)

Scer\GAL4^esg.PU, esg^HMS02538 has enteroendocrine cell | adult stage phenotype, suppressible by pros^RNAi.UAS.cUa/Scer\GAL4^esg.PU

(Li et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of sc^JF02104 or ase^{dsRNA.Scer\UAS.cUa} suppresses the increased enteroendocrine cell number seen in adult midguts of flies expressing esg^HMS02538 under the control of Scer\GAL4^Dl-05151-G.

Co-expression of pros^{dsRNA.Scer\UAS.cUa} suppresses the increased number of enteroendocrine cells seen in the adult midgut of flies expressing esg^HMS02538 under the control of Scer\GAL4^esg.PU.

(Li et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

42846