FB2024_03 , released June 25, 2024
Allele: Dmel\ppkESB
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General Information
Symbol
Dmel\ppkESB
Species
D. melanogaster
Name
FlyBase ID
FBal0281081
Feature type
allele
Associated gene
Dmel\ppk
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Boiko et al., 2012)
Mutagen
FLPase
(Boiko et al., 2012)
Progenitor genotype
PBac{WH}f07052
(Boiko et al., 2012)
P{XP}d02171
(Boiko et al., 2012)
Caused by aberration
Df(2L)ppkESB
Cytology
Description

Recombination between the two progenitor insertions has deleted the sequence between them, which includes ppk.

(Boiko et al., 2012)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Mutant larvae show defective locomotion (the degree of direction change per second is significantly reduced compared to wild type) and show a significant reduction in the response to a transient, noxious mechanical stimulus compared to wild type.

      The distinct acid-evoked transient inward Na[+] current (I[[t]]) seen in voltage-clamped wild-type class IV multidendritic (md) neurons in primary neuron cultures following acid stimulus and then removal of amiloride blockade is abolished in mutant class IV md neurons, while the sustained acid-evoked inward Na[+] current (I[[s]]) is unaffected.

      The intrinsic characteristics and firing frequency of action potentials evoked by supra-threshold depolarizing currents are similar in current-clamped class IV md neurons from both wild-type and ppkESB flies.

      (Boiko et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      ppkESB is rescued by Scer\GAL4ppk.1.9/ppkUAS.Tag:FLAG

      (Boiko et al., 2012)
      Comments

      The defects in locomotion and response to a transient, noxious mechanical stimulus seen in ppkESB larvae are rescued by expression of ppkScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ppk.1.9.

      Expression of ppkScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ppk.1.9 rescues the acid-evoked transient inward Na[+] current (I[[t]]) in ppkESB class IV multidendritic neurons in primary neuron culture.

      (Boiko et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      ppk1ESB
      (Boiko et al., 2012)
      ppkESB
      (Luedke et al., 2024, Jiang et al., 2020, Zhang et al., 2020, Guo et al., 2014)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)