FB2024_03 , released June 25, 2024
Allele: Dmel\Sema1aFc.mICD.UAS
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General Information
Symbol
Dmel\Sema1aFc.mICD.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0280760
Feature type
allele
Associated gene
Dmel\Sema1a
Associated Insertion(s)
Carried in Construct
PBac{UAS-Sema1a.Fc.mICD}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Jeong et al., 2012)
Progenitor genotype
Carried in construct
PBac{UAS-Sema1a.Fc.mICD}
Cytology
Description

UASt regulatory sequences drive expression of a chimeric protein which consists of fragment crystallizable region of human immunoglobulin G followed by the Sema1a transmembrane and intracellular domains.

(Jeong et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Sema1a
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Sema-1amICD.Scer\UAS.T:Hsap\Fc-IgG and pblScer\UAS.N.T:Ivir\HA1 under the control of Scer\GAL4elav.PLu results in ISNb guidance defects in more than 40% of hemisegments, while only mild defects in central nervous system patterning are seen.

      plexAMB09499/+ significantly suppresses the central nervous system defects seen in embryos co-expressing Sema-1amICD.Scer\UAS.T:Hsap\Fc-IgG and pblScer\UAS.N.T:Ivir\HA1 under the control of Scer\GAL4elav.PLu, while the ISNb defects are not suppressed.

      (Jeong et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Sema1aFc.mICD.UAS/Scer\GAL4sca-537.4 partially rescues Sema1ak13702

      (Jeong et al., 2012)
      Comments

      Expression of Sema-1amICD.Scer\UAS.T:Hsap\Fc-IgG under the control of Scer\GAL4sca-537.4 significantly but modestly rescues the central nervous system guidance defects seen in Sema-1ak13702 embryos.

      Co-expression of Sema-1amEC.Scer\UAS.T:Hsap\Fc-IgG,T:Hsap\MYC and Sema-1amICD.Scer\UAS.T:Hsap\Fc-IgG under the control of Scer\GAL4sca-537.4 does not result in additional rescue of the ISNb defects seen in Sema-1ak13702 embryos compared to either line expressed alone.

      (Jeong et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Sema-1amICD.Scer\UAS.T:Hsap\Fc-IgG
      Sema1aFc.mICD.UAS
      Sema1amICD.Scer\UAS.T:Hsap\Fc-IgG
      Name Synonyms
      Secondary FlyBase IDs
        References (1)