FB2024_03 , released June 25, 2024
Allele: Dmel\mir-14UAS.cLa.DsRed(Unk)
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General Information
Symbol
Dmel\mir-14UAS.cLa.DsRed(Unk)
Species
D. melanogaster
Name
FlyBase ID
FBal0280501
Feature type
allele
Associated gene
Dmel\mir-14
Associated Insertion(s)
Carried in Construct
P{UAS-DsRed-mir-14.L}
Key Links
Transgenic product class
wild_type
(Bejarano et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Bejarano et al., 2012)
Mutagen
in vitro construct
(Bejarano et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-DsRed-mir-14.L}
Cytology
Description

UAS regulatory sequences drive expression of a DsRed(Unk) fluorescent protein. A genomic fragment containing the mir-14 pre-miRNA hairpin has been inserted into the DsRed(Unk) 3'UTR.

(Bejarano et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
mir-14
Tagged with
DsRed(Unk)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Overexpression of mir-14Scer\UAS.cLa.T:Disc\RFP driven by Scer\GAL4hh-Gal4 causes curvature of the wing and significantly decreases posterior (P) wing compartment size and the posterior / anterior (A) wing size ratio compared to controls. Overexpression of mir-14Scer\UAS.cLa.T:Disc\RFP by Scer\GAL4sd.PU significantly reduces wing size. Overexpression of mir-14Scer\UAS.cLa.T:Disc\RFP at the A-P boundary by Scer\GAL4ptc.PU decreases distance between the L3 and L4 wing veins. Overexpression of mir-14Scer\UAS.cLa.T:Disc\RFP in the anterior wing compartment driven by Scer\GAL4ci.PU significantly reduces the size of the A compartment, causing it to curve in the anterior direction.

      (Kim et al., 2014)

      Animals expressing mir-14Scer\UAS.cLa.T:Disc\RFP under the control of Scer\GAL4da.G32 die at a late pupal stage with no obvious phenotypic defects in the pharate animal.

      Expression of mir-14Scer\UAS.cLa.T:Disc\RFP under the control of either Scer\GAL4ptc-559.1 or Scer\GAL4Bx-MS1096 does not result in a mutant phenotype.

      Expression of mir-14Scer\UAS.cLa.T:Disc\RFP under the control of Scer\GAL4sd-SG29.1 results in slightly curved wings.

      (Bejarano et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      mir-14UAS.cLa.DsRed(Unk)/Scer\GAL4ptc.PU is a suppressor of visible phenotype of Scer\GAL4ptc.PU, ptcJF03223

      (Kim et al., 2014)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of mir-14Scer\UAS.cLa.T:Disc\RFP suppresses the increased distance between the L3 and L4 wing veins seen in Scer\GAL4ptc.PU>ptcJF03223 flies; these flies even have a partial reduction in the distance between L3 and L4 (towards Scer\GAL4ptc.PU>mir-14Scer\UAS.cLa.T:Disc\RFP phenotypes, with a slight curving of the L4 vein towards the anterior portion of the wing).

      (Kim et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      mir-14UAS.cLa.DsRed(Unk)/Scer\GAL4btl.PU rescues mir-14Δ1

      (Kim et al., 2014)
      Comments

      Expression of mir-14Scer\UAS.cLa.T:Disc\RFP driven by Scer\GAL4btl.PU rescues the excess tracheal terminal cell phenotype seen in mir-14Δ1/mir-14Δ1 third instar larvae.

      (Kim et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      mir-14Scer\UAS.cLa.T:Disc\RFP
      mir-14UAS.cLa.DsRed(Unk)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)