FB2024_03 , released June 25, 2024
Allele: Hsap\ARFGAP1UAS.Tag:HA
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General Information
Symbol
Hsap\ARFGAP1UAS.Tag:HA
Species
H. sapiens
Name
FlyBase ID
FBal0278387
Feature type
allele
Associated gene
Hsap\ARFGAP1
Associated Insertion(s)
Carried in Construct
P{UAS-HA-hArfGAP1}
Key Links
Transgenic product class
wild_type
(Xiong et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Xiong et al., 2012)
Mutagen
in vitro construct
(Xiong et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-HA-hArfGAP1}
Cytology
Description

UASt regulatory sequences drive expression of full-length Hsap\ARFGAP1 coding sequences tagged at the N-terminal with Tag:HA.

(Xiong et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\ARFGAP1
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  Parkinson's disease
      modeled by Hsap\LRRK2G2019S.UAS.Tag:FLAG
      (Xiong et al., 2012)
      modeled by Hsap\LRRK2UAS.Tag:FLAG
      (Xiong et al., 2012)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Hsap\ARFGAP1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ple.PF results in dopaminergic neuronal loss, beginning at 5 weeks of age.

      Expression of Hsap\ARFGAP1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ple.PF mildly reduces climbing ability.

      Expression of Hsap\ARFGAP1Scer\UAS.T:Ivir\HA1 in the developing eye, under the control of Scer\GAL4GMR.PF, leads to a profound eye phenotype.

      (Xiong et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of Hsap\ARFGAP1Scer\UAS.T:Ivir\HA1 with Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG in dopaminergic neurons, under the control of Scer\GAL4ple.PF, significantly prevents the loss of dopamine neurons in PAL and PPM1/2 but not PPM3 or PPL1 clusters. Hsap\ARFGAP1Scer\UAS.T:Ivir\HA1 rescues the loss of dopaminergic neurons beginning at 5 weeks of age.

      Co-expression of Hsap\ARFGAP1Scer\UAS.T:Ivir\HA1 with Hsap\LRRK2G2019S.Scer\UAS.T:Zzzz\FLAG in dopaminergic neurons, under the control of Scer\GAL4ple.PF, significantly prevents the loss of dopamine neurons in PAL and PPM1/2 but not PPM3 or PPL1 clusters.

      Co-expression of Hsap\ARFGAP1Scer\UAS.T:Ivir\HA1 in dopaminergic neurons, under the control of Scer\GAL4ple.PF, rescues the climbing defect seen in Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG- or Hsap\LRRK2G2019S.Scer\UAS.T:Zzzz\FLAG-expressing flies.

      Co-expression of either Hsap\LRRK2Scer\UAS.T:Zzzz\FLAG or Hsap\LRRK2G2019S.Scer\UAS.T:Zzzz\FLAG rescues the eye defects seen upon expression of Hsap\ARFGAP1Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF.

      (Xiong et al., 2012)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\ARFGAP1Scer\UAS.T:Ivir\HA1
      Hsap\ARFGAP1UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (1)