FlyBase Allele Report: Dmel\mir-285[UAS.DsRed(Unk)]

General Information

Symbol

Dmel\mir-285^{UAS.DsRed(Unk)}

Species

D. melanogaster

Name

FlyBase ID

FBal0278034

Feature type

allele

Associated gene

Dmel\mir-285

Associated Insertion(s)

Carried in Construct

P{UAS-DsRed-mir-285.L}

Key Links

Transgenic product class

wild_type

(Bejarano et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Bejarano et al., 2012)

Mutagen

in vitro construct

(Bejarano et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-DsRed-mir-285.L}

Cytology

Description

UAS regulatory sequences drive expression of a DsRed(Unk) fluorescent protein. A genomic fragment containing the mir-285 pre-miRNA hairpin has been inserted into the DsRed(Unk) 3'UTR.

(Bejarano et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mir-285

Tagged with

DsRed(Unk)

red fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^ey.PU

(Li et al., 2017)

abnormal size | larval stage, with Scer\GAL4^moody.PU

(Li et al., 2017)

increased cell death | larval stage, with Scer\GAL4^en.PU

(Li et al., 2017)

increased cell death | larval stage, with Scer\GAL4^GMR.PU

(Li et al., 2017)

increased cell death | larval stage, with Scer\GAL4^hh.PU

(Li et al., 2017)

lethal, with Scer\GAL4^da.G32

(Bejarano et al., 2012)

visible, with Scer\GAL4^Bx-MS1096

(Bejarano et al., 2012, Bejarano et al., 2012)

visible | adult stage, with Scer\GAL4^en.PU

(Li et al., 2017)

visible | adult stage, with Scer\GAL4^ey.PU

(Li et al., 2017)

visible | adult stage, with Scer\GAL4^GMR.PU

(Li et al., 2017)

visible | adult stage, with Scer\GAL4^hh.PU

(Li et al., 2017)

Phenotype Manifest In

embryonic/larval brain | larval stage, with Scer\GAL4^moody.PU

(Li et al., 2017)

embryonic/larval CNS subperineurial glial cell | larval stage, with Scer\GAL4^moody.PU

(Li et al., 2017)

eye, with Scer\GAL4^ey.PU

(Li et al., 2017)

eye, with Scer\GAL4^GMR.PU

(Li et al., 2017)

eye disc, with Scer\GAL4^GMR.PU

(Li et al., 2017)

nucleus | larval stage, with Scer\GAL4^moody.PU

(Li et al., 2017)

perineurium | adult stage, with Scer\GAL4^moody.PU

(Li et al., 2017)

perineurium | larval stage, with Scer\GAL4^moody.PU

(Li et al., 2017)

perineurium | larval stage, with Scer\GAL4^repo.PU

(Li et al., 2017)

septate junction | larval stage, with Scer\GAL4^moody.PU

(Li et al., 2017)

wing, with Scer\GAL4^Bx-MS1096

(Bejarano et al., 2012, Bejarano et al., 2012)

wing, with Scer\GAL4^en.PU

(Li et al., 2017)

wing, with Scer\GAL4^hh.PU

(Li et al., 2017)

wing disc, with Scer\GAL4^en.PU

(Li et al., 2017)

wing disc, with Scer\GAL4^hh.PU

(Li et al., 2017)

Detailed Description

Statement

Reference

The expression mir-285^{UAS.DsRed(Unk)} under the control of Scer\GAL4^ey.PU induces a small eye phenotype. Expression under the control of Scer\GAL4^GMR.PU induces a rough eye phenotype associated with substantial apoptosis in the eye disc. Expression under the control of either Scer\GAL4^hh.PU or Scer\GAL4^en.PU induces wrinkled wings, associated with substantial apoptosis in the wing disc.

Expression under the control of Scer\GAL4^moody.PU induces a compromised blood-brain barrier, as dye injected into the body cavity is able to diffuse into the adult retina or third instar larval brain. In agreement, larval subperineurial glial cells show disrupted/discontinuous septate junctions (Dlg immunostaining); these cells also show significant decreases in nuclear size and in ploidy only at higher expression conditions (i.e. at 25[o]C but not at 18[o]C), as compared to controls. There are also a significant increase in larval brain volume only at higher expression conditions (i.e. at 25[o]C but not at 18[o]C), as compared to controls.

Expression under the control of Scer\GAL4^repo.PU results in a compromised larval blood-brain barrier, as injected dye into the body cavity is able to diffuse into the larval brain; no significant apoptosis is observed in the larval brain.

(Li et al., 2017)

Adults expressing mir-285^{Scer\UAS.T:Disc\RFP} under the control of Scer\GAL4^Bx-MS1096 have curved wings.

Expression of mir-285^{Scer\UAS.T:Disc\RFP} under the control of Scer\GAL4^sd-SG29.1 results in curved and folded wings which are often broken.

Expression of mir-285^{Scer\UAS.T:Disc\RFP} under the control of Scer\GAL4^ptc-559.1 does not result in a mutant phenotype.

(Bejarano et al., 2012)

Expression of mir-285^{Scer\UAS.T:Disc\RFP} under the control of Scer\GAL4^Bx-MS1096 results in wing blisters.

(Bejarano et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class